| 抗人CD80双价抗体的构建、表达及生物学功能初步研究 |
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| 引用本文： | 陈昌友,朱华亭,郭静雅,黄莉,张彦军,黄赛男,邱玉华.抗人CD80双价抗体的构建、表达及生物学功能初步研究[J].现代免疫学,2011(3):192-196. |
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| 作者姓名： | 陈昌友 朱华亭 郭静雅 黄莉 张彦军 黄赛男 邱玉华 |
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| 作者单位： | 苏州大学医学部免疫学系;苏州大学附属儿童医院呼吸科; |
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| 基金项目： | 国家科技部科技重大专项资助项目(2009ZX09103-705) |
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| 摘 要： | 构建及表达抗人CD80双价抗体(dimeric antibody fragment,diabody),并初步研究其生物学功能.PCR法获得轻重链可变区基因,构建表达载体pIRES2-EGFP/diabody.将pIRES2-EGFP/diabody转染中华仓鼠卵巢细胞(CHO),G418加压,筛选稳定高表达细胞株,扩大...
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| 关 键 词： | CD80 双价抗体 真核表达 |
Construction,expression and biological function assay of anti-human CD80 bivalent dimer |
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| CHEN Chang-you,ZHU Hua-ting,GUO Jing-ya,HUANG Li,ZHANG Yan-jun,HUANG Sai-nan,QIU Yu-hua.Construction,expression and biological function assay of anti-human CD80 bivalent dimer[J].Current Immunology,2011(3):192-196. |
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| Authors: | CHEN Chang-you ZHU Hua-ting GUO Jing-ya HUANG Li ZHANG Yan-jun HUANG Sai-nan QIU Yu-hua |
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| Institution: | CHEN Chang-you1,ZHU Hua-ting1,GUO Jing-ya1,HUANG Li2,ZHANG Yan-jun1,HUANG Sai-nan1,QIU Yu-hua1(1.Department of Immunology,Medical College of Soochow University,Suzhou 215123,China,2.Respiratory Department,Soochow University Affiliated Children's Hospital Suzhou 215003,China) |
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| Abstract: | To construct and express anti-human CD80 bivalent dimmer(diabody) and to study its biological functions,VH and VL genes were cloned by PCR,and then subcloned into the eukaryotic expression vector pIRES2-EGFP to construct recombinant pIRES2-EGFP/diabody.Chinese hamster ovary(CHO) cells was transfected by this recombinant plasmid through liposome-mediated methods and selected by G418.The anti-human CD80-diabody was purified from culture supernatants by Ni catridges.Reducing and non-reducing SDS-PAGE was used ... |
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| Keywords: | CD80 diabody eukaryotic expression |
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