应用SELDI质谱技术筛选胰腺癌患者的血清标志蛋白

目的:探讨应用SELDI质谱技术对胰腺癌患者血清中特异性标志蛋白的筛选方法。方法:采用表面增强激光解析/离子化飞行时间质谱（SELDI-TOF-MS）技术，选择WCX磁珠及蛋白芯片阅读机对胰腺癌及健康人(对照组)和胰腺良性疾病组的血清进行检测，以筛选胰腺癌患者血清中特异表达差异蛋白。结果:发现胰腺癌血清表达差异的潜在标志物（蛋白）4个，相对分子质量分别为5 705 Da，4 935 Da,5 318 Da和3243 Da，其中4 935 Da，3 243 Da差异表达蛋白质在对照组中低表达，而在胰腺癌组中高表达，5 705 Da和5 318 Da差异表达蛋白质在胰腺癌组中低表达而对照组中高表达。结论:应用SELDI技术筛选胰腺癌患者血清中的特异性生物标记物的方法快速、有效;检测到的4个差异蛋白质可能是胰腺癌患者血清特异性生物标记物。

A screening for specific biomarkers in serum of pancreatic cancer patients by SELDI technology

Abstract:Objective:To explore the methode to screen for relatively specific markers in serum of pancreatic cancer patients using surface-enhanced laser desorption and ionization time of fight mass spectrometry（SELDI-TOF-MS）.Methods :Serum samples from 20 pancreatic cancer patiants,20 healthy volunteers and 18 patients with other pancreatic diseases were examined. WCX magnetic beans and PBSⅡ-C protein chips reader (ciphergen Biosystems Ins.) were used to detect the protein fingerprint expression of all the serum samples, and the resulting profiles were analyzed with Biomarker Wizard system.Results:Four differently expressed potential biomarkers were identified with the relative molecular weights of 5 705Da,4 935Da,5 318Da and 3 243Da respectively. Compared with control group, two proteins with m/z 5 705Da and 5 318Da were down-regulated, and two proteins with m/z 4 935Da and 3 243Da were up-regulated in pancreatic cancers.Conclusions:SELDI technology is a quick, easy and practical method to screen for specific biomarkers in serum of patients with pancreatic cancer. Four different proteins identified in this study may be as specific serum biomarkers of pancreatic cancer.