A 13C NMR study of the application of [U-13C]succinate for metabolic investigations in rabbit renal proximal convoluted tubular cells |
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| Authors: | A W Jans R Willem |
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| Institution: | Max-Planck-Institut für Systemphysiologie, Dortmund 1, Federal Republic of Germany. |
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| Abstract: | The fate of the 13C label arising from U-13C]succinate in rabbit renal proximal convoluted tubular (PCT) cells, incubated with U-13C]succinate, was investigated by 13C NMR spectroscopy of the perchloric acid extracts of the cell pellets. The 13C label of U-13C]succinate was predominantly metabolized into glutamate, glutamine, and into glucose, lactate, and alanine, which is the consequence of a carbon efflux of the label from the Krebs cycle at the stage of alpha-ketoglutarate and oxaloacetate, respectively. |
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