慢性乙型肝炎患者血清HBV DNA定量检测与抗原抗体模式的关系

目的探讨慢性乙型肝炎患者血清HBV DNA含量与抗原抗体模式的关系,为慢性乙型肝炎的诊治及预防提供科学依据。方法采用荧光标记定量聚合酶链反应和酶联免疫吸附实验法检测180例慢性乙型肝炎患者血清HBV DNA含量和抗原抗体,统计分析不同抗原抗体模式HBV DNA含量。结果在HBsAg和HBeAg同时阳性模式中,HBV DNA含量>103copy.ml-1者114例(91.20)%,其中>107copy.ml-1者44例(35.20%);在HBsAg和抗-HBe同时阳性模式中,HBV DNA含量>103copy.ml-1者18例(56.25%),其中>107copy.ml-1者4例(12.50%)。HBeAg阳性模式HBV DNA含量显著高于HBeAg阴性模式(Ρ<0.01)。结论HBeAg阳性模式HBV DNA含量高于HBeAg阴性模式,HBeAg阳性模式中少数人HBV DNA含量很低,HBeAg阴性模式中少数人HBV DNA含量很高。仅根据抗原抗体模式,难以准确地判断病毒复制程度及传染性的强弱。

Relationship between the serum HBV DNA content detection and antigen-antibody mode in patients with chronic type B hepatitis

Objective To explore relationship between the serum HBV DNA content and antigen-antibody mode in patients with chronic hepatitis B(CHB).Methods Fluorescence quantitative polymerase chain reaction(PCR) was used to detect the HBV DNA content and enzyme linked immunosorbent assay(ELISA)was used to detect the antigen-antibody in 180 CHB patients.Results HBV DNA content were more than 10~3 copy·ml~(-1),and 10~7 copy·ml~(-1)(were 90.6% and 34.6% respectively)among the HBsAg and HBeAg positive patients.HBV DNA content were less than 10~3 copy/ml(35.5%),more than 10~7 copy/ml(12.9%) among the HBsAg and anti-HBe positive patients.The HBV DNA content in HBeAg positive patients were significantly higher than that in HBeAg negative patients(Ρ<0.05).Conclusion The HBV DNA content in HBeAg positive patients are significantly higher than that in HBeAg negative patients,some HBeAg positive patients' HBV DNA content are very low,and some HBeAg negative patients' HBV DNA content are very high.It is difficult to judge accurately the degrees of viral replication and infectivity according to the antigen-antibody mode.