人NGF促进感觉神经生长和诱导SP释放实验研究

目的采用体外模型对比研究观察，评估人痛性椎间盘细胞外基质对小鼠背根神经节神经轴突生长所产生的促进作用和神经肽（sP）的诱导作用。方法通过获取椎间盘源性下腰痛患者的椎间盘组织（退变组），以及正常人腰椎间盘组织（正常组）提取细胞基质，对小鼠背根神经节神经元（DRG）进行培养，通过形态学观察神经元轴突生长和酶联免疫吸附法（ELISA）检测SP含量。结果实验组神经生长因子（NGF）浓度显著高于正常组；实验组神经元轴突平均长度为（134．17±2．21）μm，明显高于正常组的（121．28±4．10）μm，二者相比有明显差异性（P〈0．05）。在退变人椎间盘组织培养液中SP物质比率明显高于正常组（P〈0．001），二者具有显著性差异。结论人退变椎间盘细胞外基质中高表达的NGF能促进感觉神经轴突的生长以及诱导和疼痛相关神经肽的释放。

Human nerve growth factor promotes sensory nerve growth and induces substance P in vitro: an experimental study

Objective To evaluate the axonal growth and induction of substance P （SP） by using rat dorsal root ganglion （DRG） neurons and degenerated human disc cells in vitro. Methods The human intervertebral discs were harvested from patients with discogenic low back pain （degeneration group） and normal people （control）. And the neurons of rat DRGs were cultured with extracted medium from human degenerative intervertebral discs. The promotion of axonal growth was observed and SP induction of DRG neurons in extracted medium was detected by immunocytochemistry. Reslnlts The concentration of nerve growth factor （NGF） in the degeneration group was significantly higher than that in the control group （P 〈 0. 005）. In degeneration group, the average length of axons was （134. 17 ±2.21）μm, which was significant longer than that of the control group （121.28±4. 10）μm. The percentage of SP-immunoreactive ceils with growing axons was significantly higher in degeneration group compared with the anti-nerve grouch factor 13 treatment and normal group （P 〈0.035）. Conclusion NGF in extracted medium from the degenerative intervertebral dics promotes the axonal growth and induces the release of SP.