化学溶石剂对兔尿路黏膜的损伤观察

目的观察两种目前已发现的化学溶石剂对兔膀胱的损伤情况。方法选取24只新西兰兔,采用自行设计的手术,建立膀胱造瘘及导尿通路,经过通路分别灌注生理盐水、EDTA-2Na、S1,48h后处死动物立即取出动物膀胱于相同部位取材两处行常规病理切片,观察黏膜病理损伤情况,统计分析比较各组间结果。结果灌注48h后,对照组无明显损伤,溶液S1组可见轻微损伤现象,较生理盐水严重(P<0.05),EDTA-2Na对黏膜损伤较为明显,与前两组比较差异有统计学意义。结论溶液S1膀胱灌注对动物黏膜损伤较为轻微,可望成为临床应用的泌尿系统化学溶石剂。

Clinical observation on the injury of urinary mucosa in rabbits caused by caculus dissolving chemical agents

Objective To evaluate the injury of urinary mucosa in rabbits caused by two caculus dissolving chemical agents. Methods A specially designed operation was performed on 24 New Zealand rabbits to establish tracts through bladder. Disodium-EDTA (EDTA-2Na group), S1 solution (S1 group) and natural saline (the control group) were irrigated trough the tracts for 48 hours respectively. The rabbits were sacrificed and the bladders were removed immediately. Two specimens from each bladder were prepared for pathological sections. The injury were evaluated and compared between the three groups. Results Irrigation with natural saline caused no significant injury, and S1 caused slight injury, showing statistically significant difference (P<0.05). Disodium-EDTA caused remarkable damage to the urinary mucosa, which was statistically different from the other two groups (P<0.05). Conclusion The injury caused by irrigation with S1 solution is slight and acceptable. Thus the agent can be a candidate of clinically used drug to dissolve urinary stones.