CD3700血细胞分析仪复检规则的建立与应用评价

目的建立CD3700血细胞分析仪的复检规则,保证血细胞分析仪检测结果的准确性,以利临床疾病的诊断和治疗。方法应用CD3700血细胞分析仪检测654份血常规标本,同时通过人工镜检法进行血细胞计数和形态学检查。对检测结果进行统计学分析,参考国际血液学复检专家组制定的"41条"规则并进行适当调整建立新的复检规则。结果根据国际血细胞分析仪复检规则进行统计分析,CD3700血细胞分析仪检测的654份标本真阳性率为24.9%,假阳性率为27.7%,真阴性率为45.6%,假阴性率为1.8%,符合率为70.5%,复检率为52.6%,漏检率为1.8%,无血液恶性肿瘤漏诊。调整新的复检规则后,重新进行统计分析,真阳性率为15.3%,假阳性率为11.0%,真阴性率为69.4%,假阴性率为3.8%,符合率为84.7%,复检率为26.3%,漏检率为3.8%,无血液恶性肿瘤漏诊。结论为了既保证血细胞分析仪的分析质量,又能减少不必要的工作负担,各临床实验室有必要根据自己所用仪器的特点制定出合理的复检规则。

Development of review criteria for Cell-Dyn 3700 hematology analyzer and its evaluation

Objective To establish review criteria for Abbott Cell-Dyn 3700 (CD3700) hematology analyzer, and to ensure the for accuracy of the detection. Methods A total of 654 samples were detected with CD3700 hematology analyzer. After that, blood smear examination was complemented to provide CBC and morphology information. The detection results were statistically analyzed. The new review criteria were established by consulting and modifying the one as recommended by an international consensus group. Results The result of 654 sample detected by CD3700 hematolegy analyzer were analyzed statistically according to the international consensus review rules. The ture positive rate, false positive rate, true negative rate, and false negative rate was 24.9%, 27.7%, 45.6%, and 1.8%, respectively. The re-test rate was 52.6%, and the missing rate was 1.8%, with no specimen of blood neoplasm missed. After the adjustment of the review rules, statistical analysis revealed a ture positive rate of 15.3%, a false positive rate of 11%, a true negative rate of 69.4%, and a false negative rate of 3.8%. The re-test rate was 26.3%, and the missing rate was 3.8%, with no specimen of blood neoplasm missed. Conclusion In order to ensure the veracity of results and reduce the workload, it is necessary for each laboratory to develop rational review criteria for blood cell counts and smear reviews based on the characteristics of the hematology analyzer.