| ELISPOT在1型糖尿病中的应用 |
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| 引用本文： | 唐维,杨琳,周智广. ELISPOT在1型糖尿病中的应用[J]. 国际内分泌代谢杂志, 2011, 31(4). DOI: 10.3760/cma.j.issn.1673-4157.2011.04.007 |
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| 作者姓名： | 唐维 杨琳 周智广 |
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| 作者单位： | 中南大学糖尿病中心,中南大学代谢内分泌研究所,中南大学湘雅二医院内分泌科,长沙,410011 |
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| 摘 要： | 酶联免疫斑点技术(ELISPOT)是在单细胞水平定性、定量检测微量抗原特异性T细胞及其分泌的细胞因子的检测技术.因其优势已替代其他T细胞检测技术,故通过对胰岛自身抗原特异性T细胞免疫标志物的检测,在阐明1型糖尿病的发病机制以及对该疾病的预测、分型诊断、判断预后和免疫治疗监测等方面都起着重要的作用.目前,关于ELISPOT的标准化和验证工作已经卓有成效,应用于临床指日可待.
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| 关 键 词： | 酶联免疫斑点 T淋巴细胞 糖尿病 |
Application of ELISPOT in type 1 diabetes mellitus |
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| TANG Wei,YANG Lin,ZHOU Zhi-guang. Application of ELISPOT in type 1 diabetes mellitus[J]. International JOurnal of Endocrinology and Metabolism, 2011, 31(4). DOI: 10.3760/cma.j.issn.1673-4157.2011.04.007 |
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| Authors: | TANG Wei YANG Lin ZHOU Zhi-guang |
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| Abstract: | Enzyme-linked immunospot assay (ELISPOT) can qualitatively and quantitatively detect antigen specific T cells and the related cytokine secretion on single cells. Because of the advantage, by detecting islet specific T cellular immunity marker, it has replaced other assays and plays a significant role in studying pathogenesis, prediction, etiological classification, prognosis and immune monitoring in therapeutic trials of type 1 diabetes. At present, the standardization and validation on the ELISPOT has been effective, the clinical application can be expected soon. |
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| Keywords: | ELISPOT T lymphocyte Diabetes mellitus |
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