miR-622在上皮性卵巢癌组织中表达及与预后的关系

目的 探讨miR-622在上皮性卵巢癌组织中表达及与预后的关系.方法 选取2010年3月至2012年3月在湖北省中医院行手术切除的上皮性卵巢癌患者81例,同期,选取行手术切除治疗的良性上皮性卵巢囊肿患者48例作为对照组,利用实时荧光定量PCR技术检测不同组织中miR-622表达,所有患者随访截止日期2017年3月31日,生存分析采用Kaplan-M eier法和Cox比例风险回归模型.结果 上皮性卵巢癌组织中miR-622相对表达量(2.16 ± 0.21),高于对照组(1.59 ± 0.15),差异有统计学意义(P<0.001);上皮性卵巢癌组织中miR-622表达量在不同FIGO分期、不同组织学分级和是否发生淋巴结转移中差异有统计学意义(P<0.05);Kaplan-M eier结果提示,高表达组患者总体生存率低于低表达组,差异有统计学意义(x2=6.932,P<0.05);FIGO分期、组织学分级、淋巴结转移和miR-622表达水平是影响患者生存的独立风险因素(P<0.05).结论 miR-622在上皮性卵巢癌组织中呈高表达,且与肿瘤发生、进展密切相关,是影响患者预后的因素.

The Expression of miR-622 in the Tissues of Epithelial Ovarian Cancer and Its Correlation with Prognosis

Objective To investigate the expression of miR-622 in the tissues of epithelial ovarian cancer and its correlation with prognosis.Methods 81 patients with epithelial ovarian cancer undergoing the surgical resection in Hubei Provincial Hospital of TCM from March of 2010 to March of 2012 were selected as the research objects. The other 48 patients with benign epithelial ovarian cysts undergoing the surgical resection over the corresponding period were selected into the control group.Real-time quantitative PCR was used to detect the expression of miR-622 in the tissues of those patients.All patients were followed up until March 31,2017.The Kaplan-Meier method and Cox proportional hazards regression model were used to perform the survival analysis.Results The relative expression level of miR-622 in the tissues of epithelial ovarian cancer was(2.16 ± 0.21),and it was significantly higher than that of the control group,which was(1.59 ± 0.15)(P<0.001).The expression of miR-622 in the tissues of epithelial ovarian cancer was significantly different between different FIGO stages,different histological grades and whether there was lymph node metastasis or not respectively(P< 0.05).The Kaplan-Meier result showed that the overall survival rate of patients in the high expression group was lower than that in the low expression group and the difference was statistically significant(x2= 6.932,P< 0.05).The FIGO staging, histological grade,lymph node metastasis and miR-622 expression level were the independent risk factors of patients'survival(P<0.05).Conclusion miR-622 was highly expressed in the tissues of epithelial ovarian cancer and was correlated closely with the occurrence and progression of the tumor,and it was an important influencing factor of patients'prognosis.