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Impaired interaction of alpha-haemoglobin-stabilising protein with alpha-globin termination mutant in a yeast two-hybrid system |
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| Authors: | Turbpaiboon Chairat Limjindaporn Thawornchai Wongwiwat Wiyada U-Pratya Yaowalak Siritanaratkul Noppadol Yenchitsomanus Pa-thai Jitrapakdee Sarawut Wilairat Prapon |
| Institution: | Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand. |
| Abstract: | Alpha-thalassaemia caused by alpha-globin gene termination codon mutations (alphaT-globin) has been explained by their inherent mRNA instability and by oxidative damage arising from the presence of membrane-bound alphaT-globin chains. To better understand the latter phenomenon, a yeast two-hybrid system was used to assay the interaction between alphaT-globin and its molecular chaperone, alpha-haemoglobin-stabilising protein (AHSP) and impaired binding of alphaT-globin with AHSP compared with alpha(wild-type)-globin was observed. |
| Keywords: | α-globin termination mutations haemoglobin Constant Spring haemoglobin Pakse α-thalassaemia α-haemoglobin-stabilising protein |
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