复合辅酶对大鼠单侧睾丸扭转复位后健侧睾丸生精功能的影响

目的：探讨复合辅酶对大鼠单侧睾丸扭转复位后健侧睾丸的组织学以及附睾精子生成的影响.方法：将30只健康雄性Wistar大鼠,随机分为假手术组（A组）、睾丸扭转复位组（B组）、复合辅酶处理组（C组）,B、C组大鼠按Turner法制作睾丸扭转模型,A组将睾丸游离后不扭转睾丸;C组于睾丸扭转复位前60 min腹腔注射复合辅酶（10 u/kg）,同时在术后注射复合辅酶10 u/（kg＆#183;d）3 d;各组喂养至术后4周时处死,取健侧睾丸标本行组织病理学观察,同时取健侧附睾尾部行精子计数及活动率检测,检测睾丸组织超氧化物歧化酶（SOD）、一氧化氮合酶（NOS）、丙二醛（MDA）水平.结果：B组、C组与A组相比生精细胞结构有破坏,精子计数以及精子活率降低,SOD、NOS、MDA水平升高;C组与B组相比,生精上皮层数增多,平均曲细精管直径（MSTD）增大,同时附睾精子计数与活动率明显升高,差异有统计学意义（P＜0.05）,且C组大鼠睾丸组织SOD水平升高,NOS、MDA水平降低,差异有统计学意义（P＜0.05）.结论：复合辅酶能够减轻大鼠单侧睾丸扭转复位后健侧睾丸组织损害,使附睾精子生成显著增多,其机制可能与减少大鼠睾丸组织内自由基生成,提高其抗自由基能力有关.

Effect of Coenzyme Complex on Rats Testicular Spermatogenetic Function of Contralateral Testicular after Unilateral Testicular Torsion Restoration

Objective ： To investigate the effect of coenzyme complex on the histological changes and spermatogenesis of contralatera/testicular after rats unilateral testicular torsion restoration. Methods： Thirty male Wistar rats were randomly divided into group A （sham-operation）, group B （testicular tor- sion group） , group C （coenzyme complex treatment group ） ,with 10 rats each. The testicular torsion model was established by the Turner method for subjects in group B and C ,testicles were dissociated with- out torsion for subjects in group A, then group C rats took the coenzyme complex treatment （10 u/kg） 60 min before testicular torsion restoration, and after surgery 10 u/（ kg ~ d） 3 d treatment. All the rats were fed under the same condition for 4 weeks and then executed. Histopathological observation was performed on the right testis. Sperm counting and activity analysis were performed in epididymis. And the level of SOD, NOS and MDA in testis were detected. Results： Comparing group B and C with group A, structure of spermatogenic cells were damaged , sperm counting and sperm viability were de- creased, level of SOD, NOS and MDA were increased ; comparing with group B, seminiferous epithelial layers of group C were increased, MSTD were enlarged , sperm concentration of epididymis and sperm viability were also increased significantly, differences had statistical significance （ P 〈 0.05 ）. Coen- zyme complex could significantly increase the level of SOD and decrease the levels of NOS and MDA, differences had statistical significance （P 〈 0. 05 ）. Conclusion： Coenzyme complex can reduce the damages of contralateral testis after unilateral testicular torsion restoration in rats. It is potent to in- crease the spermatogenesis remarkably. The mechanism is possible to be associated with reduction of free radicals and increase of anti-free radicals＇ability caused by coenzyme complex.