| siRNA干扰survivin表达对腺样囊性癌细胞株ACC-M的抑制作用 |
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| 引用本文： | 汪欣,杨军,许波,熊宇,张从纪. siRNA干扰survivin表达对腺样囊性癌细胞株ACC-M的抑制作用[J]. 重庆医学, 2008, 37(10): 1062-1064 |
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| 作者姓名： | 汪欣 杨军 许波 熊宇 张从纪 |
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| 作者单位： | 第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038;第三军医大学西南医院口腔科,重庆,400038 |
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| 摘 要： | 目的探讨靶向survivin基因RNAi对腺样囊性癌增殖的抑制效应。方法设计、合成siRNA(small interfering RNA),构建表达载体pGenesil-shRNA-survivin,将重组质粒导入人腺样囊性癌ACC-M细胞株。采用逆转录-聚合酶链反应(RT-PCR)、Western-blot法分别检测转染后的ACC-M细胞中survivin mRNA和蛋白表达的变化,用噻唑蓝(MTT)法来检测细胞的增殖。结果测序证实表达载体构建成功,在pGenesil-shRNA-survivin的ACC-M细胞株中,survivin mRNA及蛋白表达明显降低,ACC-M细胞增殖受到抑制。结论pGenesil-shRNA-survivin重组质粒能有效抑制腺样囊性癌细胞增殖。
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| 关 键 词： | RNAi 腺样囊性癌细胞株 survivin |
| 文章编号： | 1671-8348(2008)10-1062-03 |
| 修稿时间： | 2007-10-02 |
Inhibitory effect of RNAi-mediated survivin gene in adenoid cystic carcinoma cells lines ACC-M |
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| WANG Xin,YANG Jun,XU Bo,et al.. Inhibitory effect of RNAi-mediated survivin gene in adenoid cystic carcinoma cells lines ACC-M[J]. Chongqing Medical Journal, 2008, 37(10): 1062-1064 |
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| Authors: | WANG Xin YANG Jun XU Bo et al. |
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| Abstract: | Objective To investigate inhibitory effect of siRNA on survivin in Adenoid cystic carcinoma cells lines.Methods We designed and synthesized siRNA.Eukaryotic expression vector pGenesil-shRNA-survivin was constructed.Recombinant Plasmid was transfected into ACC-M cells.The expression of survivin mRNA and protein was detected by RT-PCR and Western-blot.The propagation of Adenoid cystic carcinoma cells was detected by MTT.Results The recombinant plasmid was successfully constructed.The expression of survivin mRNA and protein was obviously reduced.ACC-M cells propagation was inhibited.Conclusion Recombinant plasmid pGenesil-shRNA-survivin can specially suppress adenoid cystic carcinoma cells propagation. |
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| Keywords: | RNA interference adenoid cystic carcinoma cells lines survivin |
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