姜黄素对肝癌Hep1细胞增殖及凋亡的影响

目的进一步探讨姜黄素的抗肿瘤作用机制。方法CCK-8法检测不同浓度姜黄素作用不同时间肝癌Hepl细胞的增殖抑制率；流式细胞术检测Hepl细胞凋亡率以及各周期时相变化。结果姜黄素作用后Hepl细胞增殖抑制率明显升高，呈时间剂量效应关系；15μmol／L作用后，C0＋G1期细胞显著增多，而G2＋M期细胞显著减少，细胞凋亡率增高（P均〈0．05）。结论姜黄素可有效抑制Hepl细胞的生长和增殖，且具有明显的量效关系；其机制可能为干扰细胞周期进程，促进细胞凋亡。

Effect of curcumin on the proliferation and apoptosis of hepatocellular carcinoma Hep1 Cells

Objective To explore the anti-tumor mechanism of cureumin furtherly. Methods The proliferation inhi- bition rate of Hepl cells treated with different concentrations of eureumin for different time was observed by CCK-8 method, the apoptosis and cycle phase of which was measured by FCM. Results The proliferation inhibition rate of Hepl cell sig-nifieantly increased after the treatment of eurcumin in a dose-dependent manner. After the treatment of 15 μmol/L eureumin, cells in G0 ＋ G1 phase increased significantly, while cells in G2 ＋ M phase reduced significandy and apoptosis rate in- creased （P 〈0.05）. Conclusions Curcumin can effectively inhibit the cell growth and proliferation of Hepl in a clear dose-response relationship, and the mechanism may be that cureumin can interfere cell cycle progression and promote cell apoptosis.