吡格列酮对高糖培养的肾小球系膜细胞单核细胞趋化蛋白-1表达和合成的影响

目的观察过氧化物酶体增殖物激活受体激动剂-吡格列酮对肾小球系膜细胞单核细胞趋化蛋白-1（MCP-1）表达和合成的影响,探讨其肾脏保护机制。方法体外培养肾小球系膜细胞,随机分组为正常对照糖（NG组）、高糖（HG组）及高糖＋不同浓度吡格列酮（P1、P2、P3组）。培养48小时后收集各组细胞及上清液,应用RT-PCR方法检测细胞MCP-1mRNA含量,采用ELLSA法检测细胞上清液中MCP-1蛋白浓度。结果 （1）肾小球系膜细胞可表达及合成MCP-1;（2）与NG组比较,高糖刺激后肾小球系膜细胞MCP-1mRNA表达以及细胞上清液中MCP-1蛋白含量显著增高（P〈0.05）;（3）加入吡格列酮干预后,肾小球系膜细胞MCP-1mRNA表达以及细胞上清液中MCP-1蛋白含量显著下降,且呈现浓度依赖性。结论吡格列酮可抑制肾小球系膜细胞MCP-1mRNA表达和蛋白合成,且呈现剂量依赖性,该作用可能与其肾脏保护部分有关。

Effects of pioglitazone on expression and synthesis of monocyte chemoattractant protein-I in rat glomerular mesangial cells

Objective To observe the effects of pioglitazone on expression and synthesis of monocyte chemoattrac- rant protein-1 in rat glomerular mesangial cells（RGMCs） in Vivo and explore its reno-protective mechanisms. Methods Rat glomerular mesangial cells were cultured in the medium with normal glucose（group NG）, high glucose（group HG） and different concentrations of pioglitazone （group P1 ,P2 ,P3）. After 48h exposure, the supernatants and RGMCs were collected. RT-PCR was used to detect the expression of MCP-lmRNA,while the levels of MCP-1 protein in super- natants were measured by ELISA. Results （1） RGMCs could express and synthesize MCP-1. （2） After stimulated by high gloeuse, the levels of intracelluar MCP-1 mRNA and protein content in the supernatant were significantly increased compared with group NG. （P（0.05）. （3） Compared with group HG,the levels of intracellular MCP-lmRNA and pro- tein content in supernatants were significantly decreased in group Pl ,P2 and group P3 with a dose-dependent manner. Conclusion Pioglitazone can suppress the expression of MCP lmRNA and protein synthesis of glomerular mesangial cells in a dose-dependent manner,which may partly contribute to its reno-protection.