慢性乙肝患者e抗原血清转换与HBV基因变异的关系

目的 :了解慢性乙肝患者e抗原血清转换中HBV -DNA前C基因及C基因启动子变异的关系。方法 :HBV -DNA阳性且e抗原 /或e抗体阳性的慢性乙型肝炎患者各 30例 ,以PCR结合微板核酸杂交 -ELISA方法检测乙肝病毒前C基因 (nt 1896、nt 1899)及其基本核心启动子 (BCP ,nt 176 2、nt 176 4)变异。结果 :不论前C基因还是BCP变异 ,均与e抗原血清转换相关 (P <0 .0 5 ) ;不论e抗体阳性组还是e抗原阳性组 ,BCP变异均高于前C基因变异 (P <0 .0 5 ) ,但二者均不相关 (P >0 .0 5 )。结论 :乙肝病毒C基因启动子与前C基因的变异是随机的 ,都可下调HBeAg的表达 ,前者更易于发生变异。

ASSOCIATION OF HEPATITIS B VIRUS MUTATED GENES WITH THE HBeAg SEROCONVERSION IN PATIENTS WITH CHRONIC HEPATITIS B

Objective:To clarify the association of hepatitis B virus DNA mutants in basal core gene promoter (BCP) and in pre-c region with serum HBeAg conversion in patients with chronic hepatitis.Methods:HBV-DNA extracted by PCR from two groups of chronic hepatitis patients with serum HBV-DNA positive,including HBeAg and HBeAb positive 30 respectively, was detected to find HBV mutants in basal core gene promoter (nt 1762,nt 1764) and pre-core/core region (nt 1896,nt1899) using sandwich hybridization-ELISA assay on microplate.Results:Both of the two kinds of mutants were correlated with serum HBeAg conversion( P <0.05).BCP mutant was more often than pre-c gene mutant in both HBeAg group and HBeAb group ( P <0.05) and the two kinds of mutants were of no correlations ( P <0.05).Conclusions:HBV-DNA mutates randomly in basal core gene promoter and in pre-c gene to reduce HBeAg expressing,BCP does more easily than pre-c gene.