扁平苔藓中HtrA2/Omi与XIAP的表达及意义

[摘要] 目的：探讨凋亡相关蛋白丝氨酸蛋白酶HtrA2/Omi和XIAP在扁平苔藓皮损中的表达及其意义。方法：用免疫组化法、原位末端标记技术（TUNEL）对30例扁平苔藓患者皮损组织和30例正常人皮肤组织中HtrA2/Omi和XIAP的表达及两种组织的凋亡情况进行检测。结果：扁平苔藓组与正常对照组相比，HtrA2/Omi表达水平明显升高， XIAP表达水平明显下降，组间比较差异均有统计学意义（P < 0. 01），HtrA2/Omi的阳性表达强度与XIAP的阳性表达强度呈负相关（P<0.01）；扁平苔鲜组中细胞凋亡指数（AI）明显高于正常对照组（P < 0. 01），角质形成细胞的凋亡指数与HtrA2/Omi的阳性表达强度呈正相关（P<0.01），与XIAP的阳性表达强度呈负相关（P<0.01）。结论：HtrA2/Omi、XIAP可能通过调节扁平苔藓皮损处角质形成细胞的凋亡而参与扁平苔藓的发病。

Expression and significance of HtrA2/Omi and XIAP in lichen planus

[Abstract] Objective To investigate the expression and significance of HtrA2/Omi, XIAP in lichen planus. Methods Immunohistochemistry and TUNEL assay were used to detect the expression of HtrA2/Omi, XIAP and the apoptosis in 30 lichen planus cases and 30 normal skin cases.Results Compared with the normal skin, the expression level of HtrA2/Omi is increased and the XIAP is decreased in LP (P<0.01), there was a negative correlation between the expression of HtrA2/Omi and XIAP (P<0.01);The AI in LP is significantly higher than that in normal skin (P<0.01) , there was a positive correlation between the expression of HtrA2/Omi and AI in LP (P<0.01),but a negative correlation between the expression of XIAP and AI in LP(P<0.01).Conclusion HtrA2/Omi and XIAP may be involved in the pathogenesis of lichen planus by regulating the apoptosis of epidermal keratinocyte.