| 天龙咳喘灵中总黄酮和槲皮素含量测定简 |
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| 引用本文： | 郝建新,付志萍,王鹏,袁牧,潘俊辉,徐军. 天龙咳喘灵中总黄酮和槲皮素含量测定简[J]. 辽宁中医药大学学报, 2014, 0(1): 46-49 |
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| 作者姓名： | 郝建新 付志萍 王鹏 袁牧 潘俊辉 徐军 |
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| 作者单位： | [1]广州呼吸疾病研究所,广东广州510120 [2]广州医科大学第一附属医院,广东广州510120 [3]广州医科大学,广东广州510182 |
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| 基金项目： | 国家自然科学基金资助项目（30900576）;广州市科技计划项目（2009A1-E071） |
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| 摘 要： | 目的：测定名医验方天龙咳喘灵复方制剂中总黄酮和槲皮素的含量。方法：采用紫外一可见分光光度法测定天龙咳喘灵中总黄酮的含量；RP—HPLC测定其槲皮素的含量。结果：分光光度法测定总黄酮时，其在0．008-0．048mg·mL-1范围内线性关系良好（r=1），平均回收率为98．98％，RSD为1．65％；RP—HPLc测定槲皮素时，其在0．0168-4）．0840mg·mL-1范围内线性关系良好（r=O．9999），平均回收率为99．83％，RSD为2．03％。结论：确定了复方天龙咳喘灵总黄酮和槲皮素的含量，建立了可靠、稳定易行并且重复性较好的测定方法，可作为该方剂的质量控制标准。
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| 关 键 词： | 天龙咳喘灵 总黄酮 槲皮素 含量测定 |
Determination Contents of Total Flavonoids and Quercetin in Tianlong Kechuanling |
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| HAO Jianxin,FU Zhiping,WANG Peng,YUAN Mu,PAN Junhui,XU Jun. Determination Contents of Total Flavonoids and Quercetin in Tianlong Kechuanling[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2014, 0(1): 46-49 |
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| Authors: | HAO Jianxin FU Zhiping WANG Peng YUAN Mu PAN Junhui XU Jun |
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| Affiliation: | 1.Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, Guangdong, China; 2.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong, China; 3.Guangzhou Medical University, Guangzhou 510182, Guangdong, China ) |
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| Abstract: | Objective： To quantitate flavonoids and quercetin in Tianlong Kechuanling of polyherbal formulations. Methods ： Total flavonoids and quercetin were quantitated by UV-Visible spectrophotometry and High proficiency liquid chromatography （ HPLC ）. Results ： The standard curve of total flavonoids was linear in the range of 0.008-0.048 mg mL-1 （ r=l ）, and the average recovery ratio was 98.98%, and the RSD wasl.65%. The standard curve of quercetin was linear in the range of 0.0168-0.0840 mg mL-1 （ r=0.9999 ）, and the average recovery ratio was 99.83%, and the RSD was 2.03%. Conclusion ： We have identified and quantitated flavonoids and quercetin in Tianlong Keehuanling. The method of quantitive analysis of total flavonoids and quercetin in Tianlong Kechuanling is reliable and able to be used for the quality control of the formula. |
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| Keywords: | TianlongKechuanling total flavonoids quercetin content determination |
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