| 枸杞多糖对缺氧损伤PC12细胞保护作用 |
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| 引用本文： | 申志远,张兆辉.枸杞多糖对缺氧损伤PC12细胞保护作用[J].辽宁中医学院学报,2014(8):69-71. |
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| 作者姓名： | 申志远 张兆辉 |
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| 作者单位： | 武汉大学人民医院神经内科,湖北武汉430060 |
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| 摘 要： | 目的：研究枸杞多糖对缺氧损伤大鼠肾上腺嗜铬细胞瘤株PC12的保护作用。方法：采用氯化钴（CoCl2）建立PC12细胞,通过测定受损细胞给药后的活力、受损细胞LDH泄露量和受损细胞ROS生成量及线粒体膜电位,研究枸杞多糖对受损细胞的保护作用。结果：枸杞多糖能显著增加缺氧模型细胞的活力;降低细胞LDH泄漏量;增强缺氧损害细胞的SOD活性;并增加缺氧损害细胞线粒体膜电位。结论：枸杞多糖对缺氧损伤PC12细胞具有明显的保护作用。
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| 关 键 词： | 枸杞多糖 PC12细胞 缺氧损伤 |
Protective Effects of Polysacchrides of Lycium Barbarum on Anoxia Injury in Cultured PC12 Cells |
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| SHEN Zhiyuan,ZHANG Zhaohui.Protective Effects of Polysacchrides of Lycium Barbarum on Anoxia Injury in Cultured PC12 Cells[J].Journal of Liaoning College of Traditional Chinese Medicine,2014(8):69-71. |
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| Authors: | SHEN Zhiyuan ZHANG Zhaohui |
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| Institution: | ( Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China ) |
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| Abstract: | Objective ： To investigate the protective effect of polysacehrides of Lycium harbarum on anoxia injury in cultured PC12 cells. Methods ： Cultured PCl2 cells were treated with 150 μmol/L cobalt dichloride in combination with glucose deprivation. The protective effect of polysacchrides of Lycium barbarum on the model was evaluated by celluar survival rate, lactatedehydrogenate （ LDH ） efflux assay, content of SOD and mitoehondrial membrane potential. Results ： Lycium barbarum polysacchrides significantly increased the celluar survival rate, reduced releases of LDH, increased aetivites of SOD and mitochondrial membrane potential in PC12 cells during hypoxia injury. Conclusions：Lycium harbarum polysacchrides have a remarkably protective effect on anoxia injury in cultured PC 12 cells. |
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| Keywords: | Lycium barbarum polysacchrides PC 12 cells anoxia injury |
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