不同鸢尾属植物DPPH·药理活性作用比较

目的：研究和比较鸢尾属不同植物射干、日本鸢尾、德鸢尾清除DPPH·药理活性。方法：采用高效液相色谱法测定DPPH·含量,色谱柱：Kromasil C18（5μm,250 mm×4.6 mm）;流动相：甲醇-0.1%磷酸水（80：20）检测波长517 nm。比较各样品对DPPH·自由基清除作用。结果：射干、日本鸢尾、德鸢尾清除DPPH·的IC50值分别为0.020、0.004、0.018。三者对DPPH·自由基清除作用强弱顺序为：日本鸢尾〉德鸢尾≥射干。结论：不同鸢尾属植物对DPPH·自由基的清除作用有较大差别,提示除射干之外的其它鸢尾属植物也有较强的抗氧化作用。

Comparative Studies of Different Iris on Pharmacological Activity of DPPH

Objective： To investigate and compare the pharmacological activities of eliminating the DPPH ＂ free radical of 3 different species of Iris, Belamcanda, Iris japonica and Iris German. Method ： The content of the DPPH was measured by the HPLC method, a column of Kromasil C18 （ 4.6 mm × 250 mm, 5 μm ） was used, the mobile phase composed of methyl alcohol and 0.1%phosphoric acid solution （ 80 ： 20 ）, and the detection wavelength at 517 nm, and thus to compare the pharmacological activities of the DPPH· of the specimens. Result： The elimination of DPPH· free radical IC50 by Belamcanda,Iris japonica and Iris German were 0.020,0.004,0.018. The pharmacological activities of eliminating the DPPH· free radical were ranked as follows ： Iris japonica 〉 Iris German ≥ Belamcanda. Conclusion ： There is quite a significant difference among the different Iris species in pharmacological activity of eliminating the DPPH· free radical, which suggests that other Iris except Belamcanda also has stronger antioxidant effect.