| 高速逆流色谱分离纯化天然产物中生物碱类成分应用进展 |
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| 引用本文： | 杨莹莹,;张广晶,;张舒媛,;徐雅娟,;徐暾海,;刘铜华. 高速逆流色谱分离纯化天然产物中生物碱类成分应用进展[J]. 辽宁中医学院学报, 2014, 0(8): 100-103 |
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| 作者姓名： | 杨莹莹, 张广晶, 张舒媛, 徐雅娟, 徐暾海, 刘铜华 |
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| 作者单位： | [1]长春中医药大学,吉林长春130117; [2]北京中医药大学管理学院,北京100029; [3]吉林省中医药研究院,吉林长春130021; [4]北京中医药大学中药学院,北京100029; [5]北京中医药大学中医养生学教育部重点实验室,北京100029; [6]北京中医药大学中医养生学北京市重点实验室,北京100029 |
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| 基金项目： | 国家科技部国际科技合作项目（2010DFB33260）; 北京中医药大学创新团队项目（2011-CXTD-19） |
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| 摘 要： | 高速逆流色谱（hight-speed counter current chromatography,HSCCC）技术是国际上较新型的液-液分配色谱技术,近几年被广泛应用于天然产物的分离纯化和研制方面,在分离纯化天然产物中生物碱类化合物尤为突出。该文对高速逆流色谱技术在分离纯化天然产物生物碱类化学成分、活性成分;pH-区带精制逆流色谱在分离纯化生物碱的应用状况;以及高速逆流色谱与其他分析检测技术的联用进行了综述。
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| 关 键 词： | 高速逆流色谱 生物碱 分离 纯化 天然产物 |
Application of High-Speed Counter-Current Chromatography in Separation Andpurification for Alkaloids in Natural Products |
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| Affiliation: | YANG Yingying, ZHANG Guangjing, ZHANG Shuyuan, XU Yajuan, XU Tunhai, LIU Tonghua ( 1. Changchun University of Chinese Medicine, Changchun 130117, Jilin, China; 2. Beijing University of Chinese Medicine School of Management, Beijing 100029, China; 3. Jilin Institute of Traditional Chinese Medicine, Changchun 130021, Jilin, China; 4. Beijing University of Chinese Medicine Institute of Traditional Chinese Medicine, Beijing 100029, China; 5. Beijing University of Chinese Medicine of Traditional Chinese Medicine Health Ministry of Education Key Laboratory, Beijing 100029, China; 6. Beijing University of Chinese Medicine of Traditional Chinese Medicine Health Preserving Key Laboratory of Beijing, Beijing 100029, China ) |
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| Abstract: | High-speed counter-current chromatography （hight -speed counter current chromatography, HSCCC ）technology is a new type of international liquid-liquid distribution chromatography technology, and is widely used in recent years. The separation and purification of natural products and developed in the purification of alkaloids compounds in natural products is particularly prominent. In this paper, the high speed countereurrent chromatography in the separation and purification of natural product alkaloids chemical constituents, active ingredients; pH - zone refined counter-current chromatography in the purification of alkaloids application ; and high-speed countercurrent chromatography combined with other analysis testing technology are reviewed. |
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| Keywords: | high-speed counter-current chromatography （HSCCC） alkaloids separation purification natural products |
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