甘草药材HPLC指纹图谱研究

目的:建立甘草药材HPLC指纹图谱,并尝试将其应用到质量评价中。方法:采用RP-HPLC(DAD)法,用Agilent Extend-C18色谱柱(250 mm×4.6 mm,5μm)和Extend-C18保护柱(20 mm×4.6 mm,5μm)。以乙腈(A)-0.026%磷酸水溶液(B)为流动相。采用梯度洗脱:0~20 min,A为20%~25%;20~30 min,A为25%~33%;30~55 min,A为33%~50%;55~65 min,A为50%~60%;65~80 min,A为60%。检测波长为280 nm。结果:对29批甘草药材进行测定,标定了29个共有峰,且样品基本上被分成了三类。结论:建立了高效液相指纹图谱(RP-HPLC),能有效地体现出中药成分的复杂性;对29批甘草药材进行的相似度评价,反应出一定的差异性,推测这三类甘草的差异可能为种的不同所致。

Research on HPLC Fingerprint Spectrum of Radix Glycyrrhizae

Objective： To construct HPLC Fingerprint Spectrum of Radix Glycyrrhizae and apply it into quality assessment. Methods： By the method of HPLC-DAD, the analysis was performed on an Agilent Extend-C18 column and Extend-C18. The mobile phase was consisted of acetonitrile （A） and aqueous H3 PO4 （B）. The gradient elution of 20% - 25 % A at 0-20 rain ; 25%-33% A at 20-30 min; 33%-50% A at 30-55 min; 50%-60% A at 55-65 min; 60% A at 65-80 min. We detected the wave length was 280 nm. Results： Twenty-nine batches of Radix Glycyrrhizae were determined and 29 common peaks were demonstrated. The samples were clarified into 3 categories. Conclusion： The RP-HPLC was established to reflect the complexity of material medica; after evaluating the similarity of 29 batches of Radix Glycyrrhizae, we found there was significant difference among them, thus we speculate about these 3 categories of Radix Glycyrrhizae are different species.