| 重连口服液UPLC指纹图谱研究 |
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| 引用本文： | 韩燕全,李泽庚,洪燕,高家荣,汪永忠,李雪苓,夏伦祝.重连口服液UPLC指纹图谱研究[J].辽宁中医学院学报,2014(2):44-46. |
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| 作者姓名： | 韩燕全 李泽庚 洪燕 高家荣 汪永忠 李雪苓 夏伦祝 |
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| 作者单位： | [1]安徽中医药大学第一附属医院,国家中医药管理局中药制剂三级实验室,安徽合肥230031 [2]安徽中医药大学,安徽合肥230038 |
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| 基金项目： | 国家中医药重点学科开放基金项目（201lfbxk017B）;国家中医药重点学科建设项目（国中医药人教发[2012]32号）;安徽省高校省级自然科学研究重点项目（KJ20112226） |
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| 摘 要： | 目的：建立不同批次重连口服液uPLc指纹图谱测定方法。方法：采用UPLC测定了10批重连口服液样品。色谱条件为Waters HSS T3 C18柱（50mm×2．1mm，1．8μm）；流动相为乙腈（A）-0．05％磷酸水溶液（B），梯度洗脱；检测波长270nm；流速0．25mL·min^-1；柱温30℃。结果：建立了10批重连口服液UPLC指纹图谱，并进行了相似度比较，进一步对其中的射干苷、黄芩苷、连翘苷、黄芩素和汉黄芩素5个色谱峰进行了归属。结论：该法操作简便、重复性好，可用于重连口服液的质量评价和质量控制。
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| 关 键 词： | 超高液相色谱法 重连口服液 指纹图谱 黄芩苷 |
Study on Fingerprints of Chonglian Oral Liquid by UPLC Methods |
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| HAN Yanquan,LI Zegeng,HONG Yan,GAO Jiarong,WANG Yongzhong,LI Xueling,XIA Lunzhu.Study on Fingerprints of Chonglian Oral Liquid by UPLC Methods[J].Journal of Liaoning College of Traditional Chinese Medicine,2014(2):44-46. |
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| Authors: | HAN Yanquan LI Zegeng HONG Yan GAO Jiarong WANG Yongzhong LI Xueling XIA Lunzhu |
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| Institution: | 1.The First Affiliated Hospital of Anhui University of Chinese Medicine, Grade 3 Laboratory of TCM Preparation, State Administration of Anhui University of Chinese Medicine, Hefei 230031 , Anhui, China; 2.Anhui University of Chinese Medicine, Hefei 230038, Anhui, China) |
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| Abstract: | Objective： To establish the fingerprints of Cbonglian Oral Liquid by UPLC Methods. Metbod： UPLC was applied to detect 10 batchs Cbonglian Oral Liquid samples with a Waters HSS T3 C 18 column by acetonitrile （A） -0.05% phosphoric acid （ B ） as mobile phase for gradient elute. The flow rate was 0.25 mL · min^-1. The column temperature was at 30 ℃ and the UV detection wavelength was set at 270 nm. Result： The UPLC fingerprints of 10 batchs Cbonglian Oral Liquid samples were established and correlation coefficients of them were calculated. Furthermore, five peaks in UPLC chromatogram were identified as belamcandin, baicalin, forsythin, baicalein and wogonin, respectively. Conclusion： The method is simple and reproducible and can be used for quality control of Cbonglian Oral Liquid. |
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| Keywords: | UPLC Cbonglian Oral Liquid fingerprint baicalin |
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