| 人参中可溶性蛋白质含量测定 |
| |
| 引用本文： | 周颖,;樊荣,;张建逵.人参中可溶性蛋白质含量测定[J].辽宁中医学院学报,2014(8):95-96. |
| |
| 作者姓名： | 周颖 ;樊荣 ;张建逵 |
| |
| 作者单位： | [1]辽宁中医药大学附属医院,辽宁沈阳110032; [2]辽宁中医药大学,辽宁大连116600 |
| |
| 摘 要： | 目的：研究人参中可溶性蛋白质含量测定方法。方法：以牛血清白蛋白为对照品,采用考马斯亮蓝比色法对可溶性蛋白质含量进行测定。结果：对照品浓度在33.4~167.0μg/mL范围内具良好的线性关系（r=0.9990）,供试品溶液在60 min内稳定,平均回收率为99.74%,相对标准偏差为2.36%（n=5）。结论：本含量测定方法快速、准确,可为人参的品种选育、合理栽培和利用提供科学依据。
|
| 关 键 词： | 人参 可溶性蛋白质 考马斯亮蓝比色法 |
Determination of Soluble Protein in Ginseng |
| |
| Institution: | ZHOU Ying, FAN Rong, ZHANG Jiankui ( 1.The Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China ; 2. Liaoning University of Traditional Chinese Medicine, Dalian 116600, Liaoning, China ) |
| |
| Abstract: | Objective：To establish the content determination method of the soluble protein in ginseng. Methods ： The reference substance was bovine serum albumin （ BSA ） and Bradford method was used in this research. Results ： The linearity was fine when the reference substance concentration was between 33.4 pu g/mL to 167.0 bu g/mL （ r=0.9990 ）. The stability of sample solution was within 60 minutes. The result of the average recovery test was 99.74% with the RSD of 2.36% （ n=5 ）. Conclusion ： This determination method established was quick and accurate, which may provide the scientific basis for the breeding, rational cultivation and utilization of ginseng. |
| |
| Keywords: | ginseng soluble protein Bradford method |
| 本文献已被 维普 等数据库收录！ |
|
