吗啡依赖大鼠海马CA1区地西泮结合抑制因子基因表达与位置偏爱的观察

目的研究地西泮结合抑制因子(DBI)在大鼠吗啡精神依赖中所起的作用。方法30只雄性Sprague-Dawley(SD)大鼠随机分为研究组20只和生理盐水对照组10只,研究组再分为依赖组及戒断3d组、戒断6d组、戒断10d组。在处死前各个时点进行条件性位置偏爱测评,处死后利用组织原位杂交技术检测DBImRNA在海马CA1区(HIPCA1)的表达,进行组间比较,并用相关分析检测吗啡依赖大鼠海马CA1区DBImRNA的表达与CPP之间的相关性。结果1.吗啡成瘾组海马CA1区DBImRNA的表达OD值为0.28±0.018,显著高于对照组的0.24±0.018(P<0.05),并在戒断的第3天达到峰值,随后出现下调。2.在戒断第1、3、6、10天,研究组海马CA1区DBImRNA的表达与CPP之间呈正相关(P<0.01)。结论1.DBImRNA在慢性吗啡处理大鼠海马CA1区的表达上调,说明DBI参与了慢性吗啡依赖生物学过程。2.海马CA1区DBImRNA的表达和CPP有密切关系,推测DBI可能在精神依赖中起重要作用。

The expressions of DBI mRNA in the CA1 of hippocampus in rats after chronic morphine treatment and its CPP assessment

ObjectiveTo explore the role of diazepam binding inhibitor (DBI) in psychological dependence of rats. Methods 30 male Sprague-Dawley rats were randomly assigned to experiment group ( n =20) and control group ( n =10). The experiment group was re-classified into 4 subgroups based on the time of last morphine injection:3-hour group (dependent group), 3-day withdrawal group, 6-day withdrawal group and 10-day withdrawal group, respectively. Conditioned place preference was observed during the injection,and at day 3, 6 and 10 after the end of treatment. The mRNA levels of DBI in the region of the CA1 of hippocampus (HIPCA1) were estimated with in situ hybridization. Results(1) The mean levels of expression of the DBI in CA1 significantly increases in 3h group, reached the peak level at 3 day after the end of morphine administration group, then decreased. At the ten-day of discontinuation of treatment the expressed levels came to the baseline. (2) The expressions of CA1 are positive related to CPP. Conclusions(1)The up-regulation of DBI mRNA following chronic morphine administration and withdrawal may be the one of molecular mechanism underlying morphine dependence and withdrawal. (2) DBI may play a role in psychological dependence.