增殖核抗原和p53蛋白在大肠癌组织中的表达

目的：探讨增殖核抗原（proliferation cell nuclear antigen,PCNA)和p53蛋白表达与大肠癌组织分化的关系。方法，用免疫组织化学方法观察58例大肠癌手术标本PCNA和p53蛋白的表达情况，结果：PCNA阳性着色分布于细胞核内，癌组织PNCA阳性细胞数量明显多于正常肠组织，且分化愈差PCNA阳性的癌细胞数量愈多。P53蛋白阳性占51＝7％（30／58），但正常大肠组织均呈阴性反应，中，低分化大肠癌P53蛋白阳性率高于高分化的大肠癌，结论：PCNA阳性细胞数和P53蛋白表达与大肠癌的分化程度有关，PCNA阳性标志指数可作为判断大肠癌细胞增殖状态和估计预后的一个可靠参数。

Expression of Proliferation Cell Nuclear Antigen and p53 Protein in Colorectal Adenocarcinoma Tissues

Objective To investigate relationship between the expression of proliferation cell nuclear antigen (PCNA), p53 protein and the differentiation of colorectal adenocarcinoma.Method 58 samples of colorectal carcinoma were observed using immunohistochemical technique.Results (1) The positive staining of PCNA located in nucleus. The number of positive staining cells in adenocarcinoma tissues was much more than that of normal colorectal tissues. And the lower the differentiation of adenocarcinoma, the more the positive cells number.(2) The positive reaction of p53 protein also located in nucleus, the positive rate was 51.7% (30/58); The normal colorectal tissues showed negative reaction; The positive rates of p53 protein in low differentiated and moderate differentiated colorectal adenocarcinoma were more than that of high differentiated colorectal adenocarcinoma.Conclusion The number of positive staining cells of PCNA and the expression of p53 protein related to the differentiation of colorectal adenocarcinoma. The labeling indices of PCNA could be used as a valuable mark to understand the condition of adenocarcinoma proliferation and to estimate the prognosis.