基质金属蛋白酶及其抑制物在膀胱癌中的表达及意义

目的；探讨MMP2，MMP9及TIMP1在膀胱癌中的表达及意义。方法：采用免疫组化技术（SP法）对51例膀胱癌，10例正常膀胱组织的MMP2，MMP9和TIMP1进行检测。结合临床资料进行分析。结果：MMP2，MMP9和TIMP1的阳性表达分别为51％，25.5%和9.8%。MMP2和MMP9在高分级、高分期膀胱癌中表达的阳性率较低分级、低分期膀胱癌高（P＜0.05）。TIMP1在高分级、高分期膀胱癌中表达的阳性率较低分级、低分期膀胱癌低（P＜0.05）。MMP2和MMP9阳性表达率高，TIMP1阳性表达率低的膀胱癌患者恶性程度增加，更具有浸润和转移倾向，随访45例中死亡病例MMP2和MMP9的阳性表达明显高于存活病例，二者比较有显著性意义（P＜0.05）。而TIMP1在死亡及存活病例中的阳性表达比较差异无显著性意义（P＞0.05）。说明MMP2和MMP9阳性表达与膀胱癌的预后呈正相关。结论：MMP2，MMP9和TIMP1参与膀胱癌的浸润和转移，MMP2和MMP9可作为判断预后的指标。

The Expression and Clinical Significance of Metalloproteinases and Tissue Inhibitor of Metalloproteinase in Transitional Cell Carcinoma of Bladder

Objective: Our aim was to study expression of MMP2, MMP9, and TIMP1 in transitional cell carcinoma of bladder ( TCCB ). Methods: Fifty one specimens of TCCB and 10 normal specimens as control were assessed by immunohistochemical method ( SP method ). Results: The positive expressions of MMP2, MMP9 and TIMP1 in TCCB were 51% , 25.5%, and 9.8% respectively. The expressions of MMP2 and MMP9 in G 2 to G 3 and T 2 to T 4 stages were significantly higher than those in G 1 and T 1 stages (P<0.05 ). The expression of TIMP1 in G 2 to G 3 and T 2 to T 4 stages were significantly lower than those in G 1 and T 1 stages (P<0.05 ). The expressions of MMP2 and MMP9 tended to increase, with the tumor grade and stage and the expression of TIMP1 tended to decrease. The expressions of MMP2 and MMP9 in those cases who died were significantly higher than those survived (P<0.05 ), while the expression of TIMP1 did not change significantly. Conclusion: The expressions of MMP2 and MMP9 are useful in the prognosis of TCCB.