骨质疏松性骨折骨痂软骨细胞CD44和纤连蛋白的表达

目的:探讨骨质疏松性骨折愈合过程中胞内信号因子CD44及其配体纤连蛋白(FN)在骨痂软骨细胞的表达情况。方法:建立SD大鼠骨质疏松性股骨骨折愈合模型作为实验组以及二期股骨骨折愈合模型作为对照组,分别于骨折后10、20、30d和40d取材,对骨痂进行H-E染色及CD44、FN免疫组织化学显色。结果:在骨痂组织中CD44和FN的表达及分布在时空上有一致性。在实验组中,随着骨折愈合进程的发展,软骨细胞CD44与FN的表达逐渐减少,而对照组中,CD44与FN共同表达于骨折愈合各阶段的各区软骨细胞中。结论:大鼠骨质疏松性骨折愈合过程中骨痂软骨细胞CD44、FN的表达明显弱于正常二期骨折愈合过程中的表达,提示CD44和FN在软骨细胞中表达的明显减少可能是造成骨质疏松性骨折骨痂软骨组织向骨组织演变缓慢的原因之一。

Expression of CD44 and fibronectin in the callus cartilage cells of osteoporosis fracture

Objective:To study the expression of CD44 and fibronectin(FN)of the callus cartilage cells in the healing process of osteoporosis fracture.Methods:The fracture healing on rat femur fracture of osteoporosis was used as ex- perimental group,while another model of the second fracture healing was used as a control group.H-E staining and CD44,FN staining were applied on the callus,using the samples taken from the different stages of healing 10,20,30 and 40 days after fracture.Results:The expression and distribution of CD44 and FN were coherent in callus tissues both in time and space.In the experimental group,the expression of CD44 and FN was reduced with the healing of the fracture.CD44 and FN were together expressed in various sections of cartilage cells in different stage of healing.Conclusion:The expression of CD44 and FN of the callus cartilage cells in the healing process of rat fracture of osteoporosis is weaker than that of the sec- ond fracture healing,which hints that the apparent decrease of CD44 and FN in the cartilage cells may be one of the reasons to cause callus cartilage tissue of osteoporosis fracture change into bone substance slowly.