全反式维甲酸联合细胞因子诱导骨髓间充质干细胞分化为神经元样细胞的作用

目的 探索全反式维甲酸(ATRA)、碱性成纤维细胞生长因子(bFGF)、表皮生长因子(EGF)在诱导骨髓间充质干细胞(BMSCs)分化为神经元样细胞过程中的作用.方法 应用Ficoll分离骨髓中单核细胞,贴壁培养,观测形态并用流式细胞仪检测其细胞表面标志;用含ATRA、bFGF、EGF的条件培养基对培养的细胞向神经细胞诱导分化;采用免疫荧光染色法对分化的细胞进行鉴定.结果 分离培养的贴壁细胞具有典型的BMSCs形态和细胞表面标志,诱导后细胞表达神经元和星形胶质细胞标志神经元特异性烯醇化酶(NSE)和胶质纤维酸性蛋白(GFAP) .结论 ATRA联合细胞因子具有诱导BMSCs在体外分化为神经元样细胞的能力.

Differentiation of bone marrow mesenchymal stem cells into neurons-like cell use ATRA combined with cell factors in vitro

Objective To investigate the effect of basic fibroblast growth factor(ATRA),combined with(b-FGF)and epidermal growth factor(EGF)on inducing bone marrow mesenchymal stem cells(BMSCs)into neurons-type cell in vitro.Methods The monocyte was isolated from bone marrow with Ficoll,cultured and examined for morphology and surface markers.At last the cultured cells were induced to differetiate into neuron-like cells by ATRA,b-FGF and EGF,and the differentiated cells were identified with immunofluorescence.Results The cultured cells had the typical morphology and cell surface antigen of BMSCs and could be induced to differetiate into neuron-like cells,exhibiting the neuonral phenotype,expressing neuron specificenolase(NSE),and glial fibrillary acid protien(GFAP).Conclusion ATRA combined with b-FGF and EGF can promote the differentiation of BMSCs into neuron-like cells.