hMAM mRNA和CK19 mRNA联合检测乳腺癌骨髓微转移

目的：检测乳腺癌患者骨髓中的human mamglobin（hMAM）mRNA和CK19 mRNA表达,探讨乳腺癌患者早期骨髓微转移与临床病理学因素的关系.方法：采用逆转录-聚合酶链反应（RT-PCR一步法）法检测骨髓组织中hMAM mRNA和CK19 mRNA的表达.结果：102例乳腺癌患者骨髓hMAM mRNA、CK19 mRNA表达阳性者分别为37.3%和56.9%,均高于良性乳腺疾病患者骨髓组织中的表达（P=0.043,P=0.002）.两者的阳性表达率与肿瘤大小、临床分期和组织学分级呈显著相关,P值分别为0.000、0.001、0.020、0.003、0.031和0.001;与淋巴结转移状况、年龄和月经状况无关,P值分别为0.101、0.255、0.111、0.110、0.492和0.187.结论：乳腺癌微转移与肿瘤大小、临床分期和组织学分级有关,hMAM mRNA和CK19 mRNA均可作为检测乳腺癌骨髓微转移标志,且两者间存在一致性.

Bone marrow micrometastases of breast cancer detected by RT-PCR for hMAM and CK19

OBJECTIVE:To detect the expressions of hMAM mRNA and CK19 mRNA in bone marrow(BM)of patients with breast cancer and understand the reltionship between micrometastases in BM and clinicopathological parameters of breast cancer.METHODS:RT-PCR(one-step)was used to detect the expressions of hMAM mRNA and CK19 mRNA in BM of 102 samples of breast cancer.RESULTS:The positive expressions of hMAM(37.3%)and CK19(56.9%)in BM of breast cancer were higher than those in the BM of benign lesions of breast patients(P=0.043,P=0.002,respectively).A significant correlation was found that the positive expressions of hMAM and CK19 were related to the size of tumor,clinical stage and histological differentiation(P=0.000,0.001,0.020,0.003,0.031 and 0.001,respectively);but not related to lymph node metastasis,age or menopausal status.CONCLUSIONS:The micrometastases in BM are correlated with some pathology parameters.hMAMm and CK19 are in concordance with the dection of breast cancer micrometastasis in BM.