| HBV基因组转染对小鼠NIH3T3细胞内基质金属蛋白酶组织抑制因子的转录调控作用 |
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| 引用本文： | 谭龙益,林淑英,周付才,孔宪涛. HBV基因组转染对小鼠NIH3T3细胞内基质金属蛋白酶组织抑制因子的转录调控作用[J]. 中国免疫学杂志, 2000, 16(2): 66-69 |
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| 作者姓名： | 谭龙益 林淑英 周付才 孔宪涛 |
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| 作者单位： | 第二军医大学附属长征医院全军临床免疫中心,上海,200003 |
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| 摘 要： | ObjectiveTounderstand the mechanism of liver cirrhosis after the infection of hepatitis B virus.MethodsMousefibroblast NIH3T3 cells were transfected with 3.2 kb HBV DND by exposure of the cells tocalcium phosphate.The change of the levels of mRNA for tissue inhibitor ofmetalloproteinase 1and 2(TIMP1,2) was detected in mouse fibroblast NIH3T3 cells and the cells of transfectionwith HBV Genome by in situ hybridization.ResultsThe levels of mRNA forTIMP1 and TIMP2 were increased significantly.ConclusionHBV infection caninduce the expression of the mRNA for TIMP1 and TIMP2.
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| 关 键 词： | 基质金属蛋白酶组织抑制因子 乙型肝炎病毒 基因转染 |
| 修稿时间： | 1998-09-29 |
Transfection of HBV genome and its effect on the mRNA level of tissue inhibitor of metalloproteinase in mouse fibroblast NIH3T3 cells |
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| TAN Long Yi,LIN Shu Ying,ZHOU Fu Cai et al. Transfection of HBV genome and its effect on the mRNA level of tissue inhibitor of metalloproteinase in mouse fibroblast NIH3T3 cells[J]. Chinese Journal of Immunology, 2000, 16(2): 66-69 |
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| Authors: | TAN Long Yi LIN Shu Ying ZHOU Fu Cai et al |
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| Affiliation: | TAN Long Yi,LIN Shu Ying,ZHOU Fu Cai et al.Center of Clinial Immunology,Changzheng Hospital,The Third Military Medical College,Shanghai 200003 |
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| Abstract: | Objective:To understand the mechanism of liver cirrhosis after the infection of hepatitis B virus.Methods:Mouse fibroblast NIH3T3 cells were transfected with 3 2 kb HBV DND by exposure of the cells to calcium phosphate.The change of the levels of mRNA for tissue inhibitor of metalloproteinase 1and 2(TIMP1,2) was detected in mouse fibroblast NIH3T3 cells and the cells of transfection with HBV Genome by in situ hybridization.Results:The levels of mRNA for TIMP1 and TIMP2 were increased significantly.Conclusion:HBV infection can induce the expression of the mRNA for TIMP1 and TIMP2. |
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| Keywords: | Tissue inhibitor of metalloproteinase Hepatitis B virus Genome transfection |
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