染料木黄酮对体外人LECs增生的抑制作用

目的研究染料木黄酮对人晶状体上皮细胞（LECs）增生的影响。方法通过集落实验和四唑溴盐（MTT）比色法观察不同质量浓度染料木黄酮作用12、24、48、72h后对LECs增生的影响，流式细胞仪检测其对人LECs周期的影响及凋亡的诱导作用。结果12．5、25、50、75mg／L组对细胞集落形成均有抑制作用（P〈0．05），随质量浓度增加，集落形成率减少。作用12h、24h组质量浓度75mg／L、48h组质量浓度12．5、25、50、75mg／L以及72h组各质量浓度对LECs的抑制作用与对照组相比差异均有统计学意义（P〈0．05）。流式细胞仪结果显示随质量浓度的增大，S期细胞明显增多。作用12h、24h诱导凋亡不明显；48h组12．5mg／L组出现凋亡峰，50mg／L组凋亡明显；72h组6．25mg／L组出现凋亡，随质量浓度的增加凋亡细胞数量增加。结论不同质量浓度的染料木黄酮可以抑制LECs的增生，有剂量效应关系和时间效应关系；作用一定时间，达到一定浓度可诱导人LECs凋亡。

Antiproliferation effects of genistein on human lens epithelial cell in vitro

ObjectiveThe proliferation and migration of residual lens epithelial cells (LECs) is a critical factor of secondary cataract formation.Receptor protein-tyrosine kinase(RTPK) plays an important role in regulating the proliferation,differentiation and apoptosis of LECs.In present study,we investigated the antiproliferation effects of different concentrations of genistein,a RTPK inhibitor,on human LECs in vitro.MethodsLens epithelial cell-B3 (LEC-B3)lines were incubated with 0-75 mg/L genistein for 12-72 hours,and the survival rate was determined by tetrazolium assay(MTT).Colony test was carried out with different concentrations of genistein.Flow cytometric analysis was employed to study the influence of different concentrations of genistein on cell cycles and cell apoptosis.Results12.5,25,50 and 75 mg/L of genistein showed significant dose-dependent inhibitory effects on LEC-B3 colonies (P<0.05).The rate of colony formation was decreased gradually with the increasing of genistein concentration.75 mg/L of genistein at 12 hours and 12.5,25,50 and 75 mg/L of genistein showed statistically significant inhibition of the cell proliferation compared with the control at 24,48 hours(P=0.03).All concentrations of genistein had obvious antiproliferative effects at 72 hours.The flow cytometric analysis suggested that the LEC-B3 was blocked at the S stage.Apoptosis of LEC-B3 was found after treatment with 12.5 mg/L genistein for 48 hours and was more obvious after treatment with 50 mg/L genistein for 48 hours.The rate of apoptosis was 12.72%,51.83%,59.3% respectively in 6.25,12.5,and 25 mg/L genistein groups at 72 hours.ConclusionGenistein has an antiproliferative effect on LEC-B3 in a dose- and time-dependent manner.Certain doses of genistein can induce apoptosis of LEC-B3 with optional treating duration.