Isolation and characterization of microvessels from normal brain and brain tumors |
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| Authors: | Daniel L. Silbergeld Francis Ali-Osman |
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| Affiliation: | (1) Department of Neurological Surgery, Northwest Neuro-Oncology Research Laboratory, University of Washington Medical Center, RI-20, 1959 N. E. Pacific Street, 98195 Seattle, Washington, USA;(2) Department of Experimental Pediatrics, Section of Experimental Therapeutics, University of Texas, MD Anderson Cancer Center, 1515 Holcombe Avenue, 77030 Houston, Texas, USA;(3) Department of Neurological Surgery, University of Washington, RI-20, 1959 N.E. Pacific Street, 98195 Seattle, WA, USA |
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| Abstract: | We describe a new technique for isolating microvessels from both brain and brain tumors. This method is relatively quick and provides a microvessel preparation free of contamination by other brain tissue. Using this method, structurally intact microvessels from normal rat brain and from a malignant rat astrocytoma were isolated and characterized with light microscopy, scanning electron microrscopy and transmission electron microscopy. In contrast to microvessels derived from normal rat brain, rat astrocytoma microvessels had endothelial cells with multilayered basement membranes, extensive microvilli on the cell surfaces, and a significant increase in the number of pinocytes in the cytoplasm. Furthermore, astrocytoma microvessel endothelial cells had pleomorphic electron dense nuclei with pale perichromatin, whereas the nuclei of endothelial cells of microvessels derived from normal brain tissue were finely granular and homogeneous with characteristically electron dense perichromatin. The morphologic characteristics of the astrocytoma microvessels are similar to the histologic changes seen in astrocytoma tissue in situ, and correlate well with the known altered functions of brain tumor neovasculature. |
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| Keywords: | blood-brain-barrier brain tumors microvessels normal brain |
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