知母活性成分ZDY101调节M2受体mRNA稳定性的研究

目的探讨知母活性成分ZDY101对M2受体mRNA稳定性的调节。方法转基因细胞CHOm2在含ZDY101或溶媒的培养基中培养不同时间后，实时荧光定量PCR检测M2受体的mRNA水平；用放线菌素D抑制基因转录，测定M2受体mRNA的时相变化，求出其mRNA的半衰期，比较ZDY101组和溶媒组的差别。结果ZDY101作用一定时问后M2受体的mRNA水平高于对照组；ZDY101作用24h以上，ZDY101组M2受体的mRNA半衰期明显高于对照组。结论ZDY101能提高M2受体mRNA的稳定性，使细胞M2受体的mRNA含量升高，是ZDY101提高M2受体密度的重要机制之一。

Study on the Regulation of M2 mRNA Stability by Active Component of Zhimu,ZDY101

Objective To study the regulation of M2 receptor mRNA stability by active component of Zhimu, ZDY101. Methods After the addition of ZDY101 or vehicle to the cultured CHOm2 cells, the synthesis of M2 receptor mRNA in the cells were stopped by the addition of actinomycin D, and then the time course of the M2 receptor mRNA was quantified by real-time quantitative PCR. The slope and curve of the decline of M2 receptor mRNA was traced, and the half-life was obtained. Results The results showed that before the addition of actinomycin D, the M2 receptor mRNA level in the ZDY101 treated group was higher than the vehicle treated group. After the addition of actinomycin D, the decline of the M2 receptor mRNA was significantly slower and the half-life was significantly longer in the ZDY101 treated group than the vehicle treated group. Conclusion The increase of M2 receptor density was at least partly attributable to the increase of M2 mRNA stability caused by ZDY101.