舒肝颗粒对大鼠肝星状细胞增殖及胶原分泌的影响

目的:观察舒肝颗粒对肝星状细胞(hepatic stellate cell,HSC)增殖及胶原分泌的影响.方法:体外培养HSC-T6.设空白对照组、正常对照组和舒肝颗粒干预组,其中,舒肝颗粒干预组设7个浓度梯度.前两者加入无血清的RPMI 1640胞培养液,后者加入含舒肝颗粒的无血清RPMI 1640细胞培养液,药物终浓度分别为0.56、0.28、0.14、0.07、0.035、0.018和0.009 g/L.采用MTT法检测细胞增殖,ELISA法检测Ⅰ、Ⅲ及Ⅳ型胶原含量.结果:经48 h,舒肝颗粒干预组HSC抑制率分别为48.59%、38.24%、28.12%、21.15%、8.47%、7.26%和0.33%,明显高于正常对照组,且呈剂量效应关系.舒肝颗粒干预组细胞上清液中Ⅰ、Ⅲ、Ⅳ型胶原含量较正常对照组明显降低(5.437 μg/L±0.043 μg/L、3.26μg/L±0.217μg/L、2.18μg/L±0.245μg/L vs 13.817μg/L±0.787μg/L、8.629μg/L±0.178μg/L、5.29μg/L±0.315μg/L,均P<0.01).结论:舒肝颗粒可通过抑制HSC增殖和胶原蛋白的分泌,减少胶原纤维在肝脏内的沉积,这可能是其抗肝纤维化的作用机制之一.

Shugan Granule inhibits hepatic stellate cell proliferation and collagen production

AIM:To observe the effects of Shugan Granule on hepatic stellate cell (HSC) proliferation and collagen production.METHODS:Cultured HSC-T6 cells were divided into three groups:blank control group,normal control group and Shugan Granule intervention group.The blank control group and normal control group were cultured in serum-free RPMI 1640 medium,while the Shugan Granule intervention group was cultured in serum-free RPMI 1640 medium containing different concentrations of Shugan Granule (0.56,0.28,0.14,0.07,0...