The structural proteins of equine arteritis virus. |
| |
| Authors: | J J Zeegers B A Van der Zeijst M C Horzinek |
| |
| Affiliation: | Institute of Virology, Veterinary Faculty, State University, Utrecht, The Netherlands |
| |
| Abstract: | Equine arteritis virus (EAV) grown in Vero, BHK-21, and RK-13 cells was purified by pelleting, Sepharose 6B chromatography, and sucrose gradient centrifugation. Analysis of whole gradients by polyacrylamide slab gel electrophoresis and subsequent autoradiography revealed a large number of proteins in all fractions. Comparison of protein patterns of virus grown in the different cell systems showed that only three proteins with molecular weights of 12,000 (VP1), 14,000 (VP2), and 21,000 (VP3) were virus specific. VP1 is a phosphorylated core protein, while VP3 is a glycoprotein. These findings, together with data obtained earlier about morphology and RNA of the virion, lend further support to inclusion of EAV in the family Togaviridae with a possible relationship to lactic dehydrogenase virus. |
| |
| Keywords: | Author to whom reprint requests should be addressed. |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
