山楂叶含药血清对高脂血清诱导单核细胞与血管内皮细胞黏附能力的影响

目的研究山楂叶含药血清对高脂血清诱导的单核细胞与血管内皮细胞黏附能力及黏附分子表达的影响,并探讨其作用机制。方法采用虎红染色法检测药物血清对高脂诱导的人脐静脉内皮细胞(human umbilical vascular endothelial cells,HUVEC)和单核细胞株THP-1黏附的作用;用细胞ELISA法检测HUVEC表面细胞间黏附分子-1(intercelluar adhesion molecule-1,ICAM-1)、血管细胞黏附分子-1(vascular cellular adhesion molecule-1,VCAM-1)、P-选择素(P-selectin)的表达。结果山楂叶能抑制高脂诱导的HUVEC与THP-1的黏附,并下调HUVEC表面ICAM-1、VCAM-1、P-se-lectin的表达。结论山楂叶防治高脂血症的机制之一可能是下调血管内皮细胞黏附分子的表达,减少单核细胞与内皮细胞的黏附,从而抑制单核细胞迁移至血管内膜形成泡沫细胞而实现。

Effects of serums containing hawthorn leaf on adhesion of monocytes to vascular endothelial cell induced by hyperlipidemic serum

Objective To study the effects of serums containing hawthorn leaf (SCHL) on the adhesion of monocytes to endothelial cells induced by hyperlipidemic serum and explore the mechanism of SCHL on hyperlipemia. Methods The adhesion of monocyte cell strain THP-1 to human umbilical vascular endothelial cells (HUVES) was determined by rose Bengal stain method, and ELISA was used to detect expressions of intercellular adhesion molecule-1 (ICAM-1), vascular cellular adhesion molecule-1 (VCAM-1) and P-selectin on HUVEC surface. Results The SCHL could inhibit THP-1 to HUVEC adhesion induced by hyperlipidemic serum, and down-regulate the expression of ICAM-1, VEAM-1, P-selectin on HUVEC surface. Conclusion One mechanism of SCHL on hyperlipemia may contribute to its inhibition on arteriosclerosis by way of down-regulating the expression of vascular endothelial cells adhesion molecules, then decreasing the adhesion of monocyte to VEC, and finally inhibiting the monocytes migrating into vascular intima to develop foam cells.