钙/钙调蛋白依赖性蛋白激酶Ⅱ在大鼠生后海马发育中的表达

目的 探讨Wistar大鼠生后海马发育过程中钙/钙调蛋白依赖性蛋白激酶Ⅱ（CaMKⅡ）的表达。 方法 应用免疫荧光方法检测CaMKⅡ在生后不同时期大鼠海马CA1、CA3区和齿状回（DG）中的表达情况（n =48）。结果 CaMKⅡ于生后各期海马CA1区和DG的表达逐渐增强，生后第10天（P10）达高峰期，此后逐渐减弱；于CA3区的表达在P4和P10时均较高。其中，CaMKⅡ在CA3区的表达高于在CA1区和DG的表达，在多形层和分子层的表达高于在锥体细胞层或颗粒细胞层的表达。结论 CaMKⅡ在CA1、CA3区和DG中的表达具有特异性的时空分布模式，这可能与其在生后发育过程中的突触发生，树突、轴突形成，海马的成熟以及学习记忆功能相关。

Expression of calcium/calmodulin-dependent protein kinase Ⅱ in the hippocampal subregions of the rat during postnatal development

Objective To investigate the expression of calcium/calmodulin-dependent protein kinaseⅡ(CaMKⅡ) in the Wistar rat hippocampus during postnatal development. BR>Methods Immunofluorescent staining was applied to observe the expression of CaMKⅡ in the CA1, CA3 and dentate gyrus (DG) of the rat hippocampus during postnatal development (EM>n/EM> =48).Results In CA1 and DG, CaMKⅡ expression increased with age, reached a plateau at postnatal day 10 (P10) and then declined gradually. In CA3, CaMKⅡ expression reached a plateau at P4 and P10. The expression of CaMKⅡ in CA3 was higher than that in CA1 and DG. CaMKⅡ expression in the stratum polymorphum and stratum moleculare was much higher than that in the stratum pyramidale or granular cell layer.Conclusion The expression of CaMKⅡ has a specific temporal-spatial distribution pattern. The specific temporal spatial distribution pattern may be important to the different physiolo