人参皂苷Rh2提高人乳腺癌细胞MCF-7对5-氟尿嘧啶诱导凋亡的敏感性

目的探讨人参皂苷Rh2(ginsenoside Rh2,G-Rh2)能否提高人乳腺癌细胞MCF-7对5-氟尿嘧啶(5-fluorouracil,5-FU)的敏感性,通过协同作用扩大细胞凋亡信号。方法先用MTT法确定G-Rh2的无毒增敏质量浓度,随后将对数生长的人乳腺癌细胞MCF-7分为4组:control(不加药),G-Rh2(加入10 mg.L-1 G-Rh2),5-FU(加入25 mg.L-1 5-FU),G-Rh2+5-FU组(加入10 mg.L-1 G-Rh2和25 mg.L-15-FU),通过MTT计算联合作用指数,激光共聚焦观察细胞形态学变化,流式细胞术检测细胞周期,免疫荧光染色检测caspase-3、多聚ADP核糖聚合酶(poly ADP-ribose polymer-ase,PARP)的剪切情况。结果 MTT结果显示,G-Rh2可导致5-FU诱导的人乳腺癌细胞MCF-7死亡率增加,形态学、细胞周期检测、caspase-3和PARP凋亡蛋白免疫荧光染色结果都显示,G-Rh2+5-FU组可显著提高人乳腺癌细胞MCF-7死亡率,且引起的细胞死亡为细胞凋亡作用。结论 G-Rh2可以提高人乳腺癌细胞MCF-7对5-FU的敏感性,通过协同作用诱导细胞凋亡。

Ginsenoside Rh2 sensitized 5-fluorouracil which induced human breast cancer MCF-7 apoptosis

Objective To investigate the ginsenoside Rh2(G-Rh2)whether sensitive 5-fluorouracil(5-FU)in human breast cancer MCF cells,and expand the apoptotic signal by synergy.Methods MTT assay was used to determine the concentrations of non-toxic G-Rh2 sensitivity.Then MCF cells were divided into 4 groups.The cells in G-Rh2,5-FU,G-Rh2+5-FU groups were treated with 10 mg · L-1 G-Rh2,25 mg · L-1 5-FU and 10 mg · L-1 G-Rh2+25 mg · L-1 5-FU respectively,while those in control group were untreated.Synergistic cytotoxic effect of G-Rh2 and 5-FU was calculated.The cells were observed for morphological change by confocal microscopy,for apoptosis by flow cytometry,analyzed for breakage of caspase-3 and poly ADP-ribose polymerase(PARP)by immune fluorescent staining.Results The MTT results showed that G-Rh2 can lead to the increase of mortality in 5-FU-induced MCF-7 cells.And the morphology,cell cycle,caspase-3 and PARP apoptotic protein immunofluorescence experiments indicated that G-Rh2+5-FU group can significantly increase MCF-7 human breast cancer cell death and cell death belong to apoptosis.Conclusions G-Rh2 can sensitive 5-fluorouracil(5-FU)in MCF cells,and can induce apoptotic with the synergy effect.