RP-HPLC双波长法同时测定熟地黄中4种成分的含量

目的建立RP-HPLC双波长同时测定熟地黄中4种成分含量的方法。方法色谱柱为WatersSunfire C18柱(4.6 mm×250 mm,5μm);以乙腈-体积分数0.2%磷酸水溶液为流动相,梯度洗脱;检测波长为λ1=210 nm和λ2=330 nm;流速为1.0 mL.min-1;柱温为30℃。结果梓醇、麦角甾苷、吉奥诺苷B1和马替诺皂苷质量浓度分别在125.0～2 000.0 mg.L-1(r=0.999 9)、11.0～176.0mg.L-1(r=0.999 9)、15.0～240.0 mg.L-1(r=0.999 9)和7.5～120.0 mg.L-1(r=0.999 8)内与峰面积呈良好的线性关系。加样回收率在97.5%～101.3%内。结论该方法灵敏,操作简便,结果可靠,可用于熟地黄药材的质量控制。

Simultaneous determination of four constituents in Rehmanniae Radix Preparata by RP-HPLC under double-wave length

Objective To establish a method for determining four constituents in Rehmanniae Radix Preparata collected from different regions in China.Methods The Waters Sunfire C18(4.6 mm×250 mm,5 μm)column was used.Acetonitrile and 0.2% phosphoric acid solution were used as mobile phase gradiently at the wavelength of 210 nm and 330 nm.The flow rate was 1.0 mL · min-1 and column temperature was at 30 ℃.Results The method was developed with good linearities over the range of 125.0-2 000.0 mg · L-1(r=0.999 9),11.0-176.0 mg · L-1(r=0.999 9),15.0-240.0 mg · L-1(r=0.999 9)and 7.5-120.0 mg · L-1(r=0.999 8)of catalpol,acteoside,jionoside B1 and martynoside,respectively.And the average recoveries of the four compounds are between 97.5%-101.3%.Conclusions The established method is sensitive,convenient and reliable.It can be used for the quantification of four constituents in Rehmanniae Radix Preparata.