| 实时荧光PCR检测黄疸婴儿尿中人巨细胞病毒感染研究 |
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| 引用本文： | 陈鑫苹,符生苗,阮和球,梁才桂.实时荧光PCR检测黄疸婴儿尿中人巨细胞病毒感染研究[J].中国热带医学,2010,10(5):544-545. |
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| 作者姓名： | 陈鑫苹 符生苗 阮和球 梁才桂 |
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| 作者单位： | 海南省人民医院中心实验室,海南,海口,570311 |
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| 摘 要： | 目的探讨实时荧光PCR技术检测尿中人巨细胞病毒(HCMV)在黄疸婴儿HCMV活动性感染的检测价值。方法分别应用实时荧光PCR法和ELISA法检测2585例黄疸婴儿尿HCMV-DNA和血清HCMV-IgM,比较病毒DNA与血清抗体的平行性和敏感性。结果 2585例黄疸婴儿尿中实时荧光PCR检测HCMV-DNA阳性1428例;ELISA检测HCMV-IgM阳性1084例。两种方法阳性符合率为73.88%,实时荧光PCR法和ELISA法阳性检出率分别为55.24%和41.93%,差异有统计学意义(P0.01);实时荧光PCR法阳性标本的平均拷贝数为7.86×105。结论 HCMV是引起婴儿黄疸的主要原因之一,实时荧光PCR法检测敏感性要高于ELISA法检测,可以有效地提示体内活动性HCMV感染,具有良好的应用价值。
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| 关 键 词： | 人巨细胞病毒 HCMV—DNA 实时荧光PCR 婴儿 |
Detection of HCMV infection in urine of infants by real-time fluorescence PCR |
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| Institution: | CHEN Xin-ping, FU Sheng- miao,RUAN He-qiu,et al. (Central Laboratory,Hainan Provincial People＇s Hospital,Haikou 570311 ,Hainan,P. R. China) |
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| Abstract: | Aim To discuss the value of detection of HCMV in jaundice inants by real-time fluorescenee PCR. Methods Real-time fluorescenee PCR and ELISA were used to detect HCMV-DNA in urine and HCMV-IgM respectively, in serum of 2585 jaundice infants. Results There 1 428 cases positive for HCMV-DNA were detected with a eoineidenee rate of detection was 73.88% and HCMV-IgM was detected in 1084 cases. The psoitive rates of RT-PCR and ELISA were 55.24% and 41.93% ,showing significant difference （P〈0.01） The average copies of RT-PCR was 7.86×10^5. Conclusion HCMV is one of the main pathogen for infant jaundice, the sensitivity of real-time fluorescence PCR is higher than the ELISA. |
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| Keywords: | HCMV-DNA |
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