| 蚊体内马来丝虫幼虫的基因检测 |
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| 引用本文： | 陈锡欣,黄炳成,刘慎良,韩广东,刘新. 蚊体内马来丝虫幼虫的基因检测[J]. 中国媒介生物学及控制杂志, 2001, 12(2): 130-131 |
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| 作者姓名： | 陈锡欣 黄炳成 刘慎良 韩广东 刘新 |
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| 作者单位： | 1. 山东省寄生虫病防治研究所(济宁 272033)
2. 山东省枣庄市卫生防疫站 |
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| 摘 要： | 目的：探讨蚊体内马来丝虫幼虫基因检测的新方法，并用于丝虫病监测。方法：利用基因工程技术合成马来丝虫寡核苷酸片段，经32P标记后作为探针，以斑点杂交法检测蚊体内马来丝虫幼虫。结果：该探针可从多种标本中特异地检出马来幼丝虫DNA，敏感性为2ng靶DNA量，蚊体内含1条幼虫即可出现阳性杂交。结论：该技术具有良好的实用性，可用于蚊体内马来丝虫幼虫的基因检测。
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| 关 键 词： | 基因检测 探针 马来幼丝虫 蚊体 丝虫病 |
| 修稿时间： | 2000-06-05 |
Studies on Gene Detectionof Brugia malayi Larvae in Mosquito |
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| CHEN Xi-xin ,HUANG Bing-cheng,LIU Shen-liang. Studies on Gene Detectionof Brugia malayi Larvae in Mosquito[J]. Chinese Journal of Vector Biology and Control, 2001, 12(2): 130-131 |
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| Authors: | CHEN Xi-xin HUANG Bing-cheng LIU Shen-liang |
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| Affiliation: | CHEN Xi-xin *,HUANG Bing-cheng,LIU Shen-liang |
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| Abstract: | Objective:To establish a new method for gene detection of Brugia malayi larvae in mosquito and use it in filariasis surveillance. Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32 P and used as probe. The B.malayi larvae in mosquito was detected with dot blot. Results: Brugia malayi larvae DNA could be specifically detected in different samples with this probe and the sensitivity was 2 ng target DNA of B.malayi . Even only one larvae in mosquito could be detected after hybridization. Conclusions:This method is practicable and reliable for gene detection of Brugia malayi larvae in mosquito. |
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| Keywords: | Gene detection Probe Brugia malayi larvae |
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