高功率微波辐照后大鼠卵巢组织Bcl-2及C-myc蛋白的表达

 目的检测高功率微波(High power microwave,HPM)与细胞凋亡相关基因Bcl-2和C-myc蛋白的关系.方法健康成年雌性SD大鼠100只,随机分为20组,每组5只.以S波段平均功率密度分别为20 mW/cm2的HPM辐照实验组大鼠10min,40mW/cm2辐照5min、10min.照后6 h、24h、48h、72 h、5 d取卵巢组织,应用免疫组织化学S-P法检测Bcl-2和C-myc蛋白表达的改变.结果HPM辐照后,24h组Bcl-2和C-myc蛋白表达升高,40mW/m2组二者升高较20mW/cm2组升高明显,差异有显著性意义(P＜0.01),正常对照组无表达.结论HPM辐照可促进Bcl-2和C-myc蛋白的活化和表达,可能是HPM诱导凋亡的机制之一.

Effects of high power microwave on expressions of Bcl-2 protein and C-myc protein in rat ovarian tissue

Objective To investigate the changes in the expressions of B cl-2 protein and C-myc protein in ovaries of rats exposed to high power microw a ve(HPM) to elucidate the possible mechanisms underlying the apoptosis induced by HPM at genetic translation level.Methods One hundred healthy female SD rats were randomized into unexposed control group and experimental groups . The experimental groups were irradiated with S wave band 20 mW/cm2, HPW for 10 min,and 40 mW/cm2 HPW for 5 min and 10 min,separately.Samples of ovarian tissue were taken 6 h, 24 h, 48 h, 72 h and 5 d after irradiation and s tudied separately. Five blank radiati on groups served as controls.Then immunohistochemical S-P staining was perform ed to assay the expressions of Bcl-2 protein and C-myc protein in germ cells o f rats.Results The expressions of Bcl-2 protein and C-myc prote in in 24 h groups were up-regulated after irradiation with HPM for 5 min and 10 min,and were higher in 40 mW/cm2 groups than in 20 mW/cm2 groups ( P<0.01).There was no expressions of C-myc / Bcl-2 proteins in the co ntrol groups.Conclusions Irradiation with high power microwave f or 24 h can induce up-regulation of C-myc protein and Bcl-2 protein expressio ns in rat ovarian cells, which is one of the mechanisms of the apoptosis induced by HPM.