| 实时荧光PCR检测宫颈组织TSLC1基因表达 |
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| 引用本文： | 唐振华,李柱南,王进进,胡伟平,黄勇. 实时荧光PCR检测宫颈组织TSLC1基因表达[J]. 上海医学检验杂志, 2010, 0(3) |
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| 作者姓名： | 唐振华 李柱南 王进进 胡伟平 黄勇 |
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| 作者单位： | 中国福利会国际和平妇幼保健院; |
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| 摘 要： | 目的建立检测肺癌肿瘤抑制因子1(TSLC1)基因mRNA表达的实时荧光聚合酶链反应(PCR)方法。方法设计特异性引物和TaqM an探针,以3-磷酸甘油醛脱氢酶(GAPDH)基因作为对照,建立检测TSLC1基因表达的实时荧光PCR方法,并采用细胞株和宫颈组织样本进行验证。结果该方法检测TSLC1基因mRNA的检测下限为每反应10个细胞或2拷贝cDNA,总不精密度为3.1%。TSLC1基因mRNA在子宫肌瘤、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)与宫颈癌患者宫颈组织中的表达率分别为100%、75%、55%和24%。宫颈癌细胞株中未检出TSLC1基因mRNA表达。所有样本中均有GAPDH基因mRNA表达。结论该实时荧光PCR方法能够有效检测TSLC1基因mRNA表达,是进一步研究TSLC1基因在宫颈癌发生中的作用及宫颈癌早期诊断的重要工具。
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| 关 键 词： | 实时荧光聚合酶链反应 TSLC1基因 宫颈癌 |
Expression of TSLC1 gene in cervical tissue by real-time fluorescence PCR |
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| TANG Zhenhua,LI Zhunan,WANG Jinjin,HU Weiping,HUANG Yong.. Expression of TSLC1 gene in cervical tissue by real-time fluorescence PCR[J]. Shanghai Journal of Medical Laboratory Sciences, 2010, 0(3) |
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| Authors: | TANG Zhenhua LI Zhunan WANG Jinjin HU Weiping HUANG Yong. |
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| Affiliation: | TANG Zhenhua,LI Zhunan,WANG Jinjin,HU Weiping,HUANG Yong.(The International Peace Maternity , Child Health Hospital,the China Welfare Institute,Shanghai 200030,China) |
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| Abstract: | Objective To establish real-time fluorescence polymerase chain reaction(PCR) method for the detection of tumor suppressor in lung cancer 1(TSLC1) mRNA expression.Methods The real-time fluorescence PCR method for the detection of TSLC1 gene expression was established with designed specific primers,TaqMan probe and glyceraldehyde 3-phosphate dehydrogenase(GAPDH) gene as control,and verified by cell lines and cervical tissue samples.Results The detection limit of TSLC1 gene mRNA was 10 cells per reaction,or 2 ... |
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| Keywords: | Real-time fluorescence polymerase chain reaction TSLC1 gene Cervical cancer |
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