消癌平注射液的抗胃癌作用(英文)

目的:消癌平是传统用于治疗咳嗽和哮喘的萝藦科中药通光藤的提取物,具有抗炎和抗肿瘤作用。通过体内外研究消癌平对人胃癌SGC-7901细胞诱导凋亡作用,而探讨其抗肿瘤活性。方法采用消癌平药物剂量2040mg·mL1作用SGC-7901细胞24和48h。体外实验采用MTT检测法评估药物对细胞生长和细胞活力,流式细胞仪检测药物对SGC-7901细胞凋亡和周期阻滞作用,体内实验采用裸鼠移植瘤模型检测其体内抗肿瘤活性,免疫组化和细胞凋亡TUNEL法检测药物对荷瘤鼠体内肿瘤组织中的凋亡蛋白Caspase-3,Bax和Bcl-2的表达。结果:消癌平可以显著抑制SGC-7901细胞的生长,并存在时间和剂量依赖性性,经过24h药物处理后其IC50约在(38.20±0.27)mg·mL1。流式细胞仪分析消癌平可诱导SGC-7901细胞凋亡和周期阻滞实验显示其可将SGC-7901细胞周期阻滞于G1。体内实验显示,经消癌平治疗组的肿瘤体积和重量都存在显着降低,中剂量组和高剂量组的抑瘤率分别为61.19%和69.07%。免疫组化和细胞凋亡TUNEL法检测显示,消癌平治疗组小鼠肿瘤组织中的Bax和caspase-3蛋白表达量显著增加,而Bcl-2的表达量下降。结论:消癌平在体外和体内对人胃癌SGC-7901细胞均有抗肿瘤活性,并可能通过诱导SGC-7901细胞内凋亡蛋白Bax和caspase-3蛋白的表达这一机制发挥抗肿瘤作用。

Antitumor activity of Xiaoaiping injection on human gastric cancer SGC-7901 cells

Xiaoaiping, extracted from Marsdenia tenacissima （Asclepiadaceae）, is a Chinese medicine used to treat cough and asthma. Previous studies have shown that its active ingredients possess anti-inflammatory and anticancer effects. The aim of this study was to investigate the antitumor activities of Xiaoaiping through apoptosis induction in human gastric cancer SGC-7901 ceils. The MTT assay was used to assess the cell growth and cell viability. SGC-7901 cells were treated with Xiaoaiping injection （20-40） mg.mL-1] for 24 and 48 h. The apoptotic cells and the cell cycle distribution were analyzed by flow cytometry. The in vivo activity of Xiaoaiping was determined by the growth inhibition of the established tumor xenografts in nude mice. Caspase-3 activity, Bax and Bcl-2 proteins in tumor tissue were measured by immunohistochemistry and apoptosis was assayed by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling （TUNEL） method. Xiaoaiping inhibited SGC-7901 cell growth in a time and dose dependent-manner and the estimated ICs0 was （38.20 ± 0.27） mg.mL-1 after 24 h of treatment. The body weight and the tumor volume were significantly reduced in nude mice beating human gastric tumor treated with Xiaoaiping. The inhibition rate of tumor growth in the mid-dose （200 mg.kg-1） group and high dose （400 mg.kg-~） group were 61.19% and 69.07%, respectively. Immu- nohistochemical staining showed an increase in caspase-3 and Bax expression whereas Bcl-2 expression decreased gradually. Xiaoaip- ing exerted potent antitumor activity in vitro and in vivo against human SGC-7901 cells; induced apoptosis and G~ cell cycle arrest. These results suggest that Xiaoaiping is a promising antitumor agent for the treatment of human gastric cancer.