海洋来源真菌草酸青霉(Penicillium oxalicum)次级代谢产物的分离与鉴定

目的研究海洋来源真菌草酸青霉(Penicillium oxalicum)的次级代谢产物。方法采用重结晶、硅胶柱色谱、Sephadex LH-20柱色谱、开放ODS柱色谱等方法进行分离纯化;根据理化性质及光谱数据确定化合物的结构。结果分离得到10个化合物,分别鉴定为N-acetyl-hydrazinobenzoic acid(1)、N-2-cis-(4-hydroxyphenyl)ethenyl]formamide(2)、p-hydroxyphenylacetamide(3)、penipanoid A(4)、penipanoid C(5)、2-(4-hydroxybenzyl)quinazolin-4(3H)-one(6)、oxaline(7)、meleagrin(8)、(3R,4R)-3,4,8-trihydroxy-3,4-dihydro-1(2H)-naphthalenone(9)和(3R,4R)-3,4,6,8-tetrahydroxy-3,4-dihydro-1(2H)-naphthalenone(10)。结论化合物1为新天然产物,化合物9、10为首次从青霉属真菌中分离得到。

Isolation and identification of the secondary metabolites from the marine-derived fungus Penicillium oxalicum

Objective To study the secondary metabolites from a marine-derived fungus Penicillium oxalicum. Methods The compounds were isolated and purified by recrystallization, silica gel, Sephadex LH-20, and ODS column chromatography, and their chemical structures were elucidated by their physico-chemical properties and spectral analysis. Results Ten compounds were obtained and identified as N-acetyl-hydrazinobenzoic acid (1), N-2-cis-(4-hydroxyphenyl)ethenyl]formamide (2), p-hydroxyphenylacetamide (3), penipanoid A (4), penipanoid C (5), 2-(4-hydroxybenzyl)quinazolin-4(3H)-one (6), oxaline (7), meleagrin (8), (3R, 4R)-3, 4, 8-trihydroxy-3, 4-dihydro-1(2H)-naphthalenone (9), and (3R, 4R)-3, 4, 6, 8-tetrahydroxy-3, 4-dihydro-1(2H)-naphthalenone (10). Conclusions Compound 1 is a new natural product, and compounds 9-10 are isolated from Penicillium genus for the first time.