Characterization of virus-primed CD8+ T cells with a type 1 cytokine profile |
| |
Authors: | Christensen, Jan Pravsgaard Stenvang, Jens Peter Marker, Ole Thomsen, Allan Randrup |
| |
Affiliation: | Institute of Medical Microbiology and Immunology, The Panum Institute 3C Blegdamsvej, 2200 N, Copenhagen, Denmark 1 The Bartholin Institute, Kommunehospitalet 5Øster Farimagsgade, 1399K Copenhagen, Denmark |
| |
Abstract: | Infection with lymphocytic chorlomeningitis virus is associatedwith marked polyclonal activation of the CD8+ T cell subpopulation.In this report the cytokine production of virus-activated Tcells is analyzed and the producing cells subset is characterizedphenotypically. CoinciB. A. Askonasding with other parametersof cell-mediated immunity, splenic T cells appear which areable to release high amounts of IFN-, but not IL-5, IL-10 ortumor necrosis factor- upon short-term stimulation with anti-CD3in vitro. A similar profile is observed analyzing T cells takenfrom an inflammatory site. Phenotypically, the main cytokine-producingcell subset is found to be CD8+ cells targeted for homing toinflammatory sites (VLA-4hiL-selectinlo) of which 30–40%were positive by intracellular staining for IFN-. This subsetalso contains all T cells with a cytotoxic potential as measuredby redirected killing. An enhanced cytotoxic potential as wellas an increased capacity to produce IFN- is observed for atleast 2 months after infection and cell sorting analysis revealedthat this could be ascribed to a long-standing increase in thefrequency of CD8+Pgp-1hi cells. Therefore, these results demonstratethat systemic virus infection may exert marked perturbationof the CD8+ T cell population resulting in generation of a long-livedsubset of primed cells with important effector potential. |
| |
Keywords: | CD8+ T cells cytokines inflammation virus infection |
本文献已被 Oxford 等数据库收录! |
|