丹参多酚酸纯化工艺研究

目的：优选丹参多酚酸的纯化工艺条件。方法：采用高效液相色谱法，以丹酚酸B的含量为指标，对絮凝剂和大孔吸附树脂纯化条件进行了考察。结果：最佳工艺条件丹参多酚酸提取液按每克药材加入约1％壳聚糖醋酸溶液1ml，絮凝2h，萃取pH为2～3，采用D101大孔吸附树脂，上样量药材：树脂为0．5g：1g，以5BV50％乙醇溶液洗脱，丹参酸B含量达85．50％。结论：该工艺可较好的纯化富集丹参多酚酸有效成分。

Study on the Preparative Purification of Salvianolie Acid from Salvia Mihiorrhiza

Objective ： To study the preparative purification of salvianolie acid from salvia mihiorrhiza. Method： The optimum preparative purification of macroreticular resin and flocculant was evaluated by measuring the content of Salvianolic acid B by HPLC. Restilt： The optimum conditions were screened,which were lml 1% chitosan corresponding to 1g salvia mihiorrhiza, and flocculated for 2h, and the volume of 50% （V/V） ethanol as eluant was 5BV. By this method, the elution efficiency of salvianolic acid B exceeded 90%. Conclusion： The method is more effective for large-scale isolation and purification of salvianolie acid from salvia mihiorrhiza.