加替沙星与牛血清白蛋白相互作用的研究

目的研究不同酸度条件下, 加替沙星与牛血清白蛋白之间的相互作用.方法采用荧光光谱和紫外光谱法进行研究.结果运用荧光猝灭双倒数图计算了在不同条件下二者的结合常数K, 根据Foster非辐射理论计算在正常生理条件下二者的结合距离r, 并通过热力学参数确定了二者的作用力类型.结论加替沙星与牛血清白蛋白之间有较强的相互作用, 以电荷作用力为主.

Interaction Between Gatifloxacin and Bovine Serum Albumin

Aim To study the reaction mechanism between gati fl oxacin and bovine serum albumin (BSA) at different pHs. Methods Fluorescence spectra and UV absorbance spectra were used. Results The binding constants were determined from a double reciprocal Lineweaver- Burk curves at different pHs. The binding distance r under normal physiologi cal condition was obtained according to Foster theory of non-radiative energy t ransfer. The binding force between gatifloxacin and BSA was inferred by thermody namical coordination. Conclusion The interaction between gatifl oxacin and BSA seems to be strong and the main binding force is electrostatic fo rce.