内耳免疫反应时血清及外淋巴液IL-6水平的测定及其意义

为了解在用ｋｅｙｈｏｌｅ ｌｉｍｐｅｔ ｈｅｍｏｃｙａｎｉｎ引发的大鼠继发性内耳免疫反应时，实验动物血清及外淋巴液中是否有细胞因子活性的变化，采用免疫测定方法对实验动物血清及外淋巴液中白细胞介素活性进行测定。结果；在发生继发性内耳反应初始，外淋巴液未检测到ＩＬ－６，而６ｈ后外淋巴液中检测到了ＩＬ－６的最早水平，２４后ＩＬ－６水平达到最高峰。

Determination of IL-6 in serum and perilymph during inner ear immune response

During secondary iuner ear immune responses against keyhole limpet hemocyanin (KLH),to understand the change of interleukin-6 (IL-6) levels in perilymph and serum, IL-6 lev-els in perilymph and serum were investigated using an immunoassay. No IL-6 levels were detectable at Day o in perilymph. The earliest perilymph IL-6 levels were observed after 6 h,peaking at 24 h,and then decreasing gradually. IL-6 level reninants were detected at 7 days in perilymph. In con-trast,during this observatinn period,low IL-6 levels were only detectable in perilymph from control ears,disappearing after 72 h. IL-6 levels dont change in serum. Previous study has identified the endolymphatic sac (ES) lags behind the earlier appearance of intercellular adhesion molecule-l (I-CAM-1) in the spiral modiolar vein and spiral ligament during immune response. The present study not only provides further support for the existence of an inner ear immune response which is regu-lated by cytokines,but also supports that cytokines controlling expression of adhesion molecules are released probably by cells which located out of ES.