沙苑子黄酮对DMN诱导的大鼠肝纤维化形成的影响

目的　观察沙苑子黄酮对二甲基亚硝胺 (DMN)诱导的大鼠肝纤维化形成的影响。方法　采用DMN诱导大鼠肝纤维化 ,期间给予灌服沙苑子黄酮 (30 ,6 0 ,12 0mg·kg-1) ,设秋水仙碱组作对照 ,给药 4wk后 ,测定血清谷丙氨转氨酶(ALT)、谷草转氨酶 (AST)活性、总蛋白 (TP)和白蛋白 (Alb)含量 ,放射免疫分析法测定血清透明质酸 (HA)、层粘连蛋白(LN)含量。光镜和电镜观察肝细胞结构和肝纤维化程度。结果　沙苑子黄酮预防给药 ,明显降低DMN诱导肝纤维化大鼠血清ALT、AST活性 (P <0 0 1,P <0 0 1) ,提高血清TP和Alb含量 (P <0 0 5 ,P <0 0 1) ,降低血清HA和LN含量 (P <0 0 1)。病理学观察结果 ,沙苑子黄酮预防组大鼠胶原纤维沉积明显减轻 ,假小叶结构明显减少 ,电镜观察显示 ,沙苑子黄酮组肝细胞结构接近正常 ,disse间隙、肝细胞内胶原纤维明显减少。结论　沙苑子黄酮预防给药 ,可抑制DMN诱导的大鼠肝纤维化形成

Effect of flavonoids of Astragali complanali on liver librosis induced by DMN in rats

AIM To observe the preventive effect of flavonoids from Astragali complanali(FAC) on liver fibrosis in rats. METHODS The liver fibrosis model in rats was established by using DMN. Rats were treated with Saline, FAC (30,60,120 mg·kg -1 three dosage groups) and Colchicine(0 1 mg·kg -1 ). The activities of ALT, AST and contents of TP, Alb, HA and LN in rat serum were measured. The histopathological changes were observed by light microscopy and electromicroscopy. RESULTS In the FAC preventive groups, serum levels of ALT and AST in DMN rats were reduced ( P< 0 01, P< 0 01). The contents of Alb and TP were increased significantly ( P< 0 05, P< 0 01). The serum levels of HA and LN were remarkably reduced in the low, middle and high dose groups( P< 0 01)\ of\ FAC preventive groups and Colchicine group, especially in middle and high doses. The pathological improvements were observed. In preventive groups of FAC, degrees of inflammatory activity and fibrogenesis in liver tissues were markedly reduced respectively compared with model group. The formation of liver pseudoluboli was alleviated. Ultrastructurally, cell organelles were restored to normal generally and few collagenous fibers were seen in Disse space in the rats of preventive group. CONCLUSION FAC can inhibit apparently liver fibrosis in DMN rats.